The invention relates to an isatis root, lucid ganoderma and rhodiola composition capable of improving microcirculation and preparations thereof. The composition is composed of 25-35 parts of isatis roots, 20-30 parts of lucid ganoderma, 10-25 parts of rhodiola, 0-10 parts of liquorice and 0-5 parts of wormwood. The preparations such as external use liquid medicine, external unguent, internal instant herbal medicine, granules, powder, tablets, capsules, water pill agents and concentrated soluations are formed through a common extraction technology. According to the isatis root, lucid ganoderma and rhodiola composition, the isatis roots are matched with the lucid ganoderma and the rhodiola appropriately. The composition is safe, free of toxicity and capable of being used for cold prevention, herpes simplex virus infection resisting, burn and scald treatment and treatment of other acute diseases; the composition can also be used for a long term, the human body microcirculation improving effects such as toxin generation restraining and harmful metabolite removing can further be achieved, and the effect that one drug can be used for treating various diseases and the anti-aging effect are achieved.