Sulphonated Gleditia sinensis Lam polysaccharide and application thereof
A technology of sulfation and acacia, which is applied in the directions of medical preparations containing active ingredients, organic active ingredients, antiviral agents, etc., can solve the problems of high and low anti-HIV activity, and the sulfated acacia polysaccharide has not yet been reported.
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Embodiment 1
[0019] Embodiment one: a kind of preparation method of compound of the present invention
[0020] Take 80 mL of sulfonating agent (chlorosulfonic acid: pyridine = 1: 8) and place it in a 250 mL three-necked flask, control the temperature at 75° C., add 8 grams of acacia (Gleditiasinensis Lam) polysaccharide, stir and react for 12 hours, take out the flask after the reaction and cool to room temperature. Neutralize with 6M NaOH solution, precipitate with ethanol, centrifuge, dissolve the precipitate in distilled water and dialyze in distilled water, distill and concentrate the inner liquid of the dialysate under reduced pressure, precipitate with ethanol, centrifuge, and dry to obtain the saponins polysaccharide sulfate ester of the present invention. The molecular weight of the product is 7.9 ×10 4 Daltons, sulfation degree 1.0. Analysis by HPLC showed that the sulfated saponins polysaccharide was composed of galactose and mannose, and the composition ratio was 1:4. Accordin...
Embodiment 2
[0021] Embodiment two: the pharmacological effect of sulfated saponins of the present invention
[0022] 1. Anti-HIV-1IIIB (X4 virus) activity of sulfated saponins polysaccharides in vitro
[0023] On a 96-well culture plate, make 5-fold dilutions of the stock solution of the sulphated saponins to be tested, and make 8 gradients in total, with 4 wells for each gradient, 100 μl per well, and set AZT positive control group and blank control group at the same time . Then, 50 μl (2×104 / well) of C8166 cells (a T lymphocyte line, donated by British Medical Research Council (MRC), AIDS Reagent Project) and HIV-1 cells were added to each well. IIIB (H9 / HIV-1IIIB, donated by the British MRC, resuscitated, cultured, collected, aliquoted and frozen according to conventional methods) virus 20 μl (MOI value 0.2), supplemented with complete medium to make the final volume of each well 200 μl. 37°C, 5% CO 2 to cultivate. On the third day, 100 μl of fresh complete medium (RPMI-1640) was a...
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