Preparation method for blattodea polypeptide substance, and medical use of blattodea polypeptide substance in anti-herpesvirus
A herpes simplex virus and cockroach technology, applied in the field of medicine, can solve the problems of inactive substances in the prevention and treatment of virus-infected diseases, such as literature reports, to achieve potential social and economic benefits, facilitate industrialization, and increase income
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Embodiment 1
[0024] Example 1 : Preparation of Periplaneta americana aqueous extract enriched by ultrafiltration membrane separation method for effective fractions of polypeptides with a molecular weight less than 5KDa (sample A)
[0025] 1.1 Preparation of Periplaneta americana Water Extract
[0026] Add 3 kg of Periplaneta americana medicinal materials, add 10,000 ml of double distilled water, soak and extract once at 45°C for 24 hours; soak and extract once at 50°C for 24 hours; soak and extract once at 55°C for 24 hours. The extracts were combined, concentrated to dryness under reduced pressure, and dried in vacuum to obtain the aqueous extract of Periplaneta americana.
[0027] 1.2 Preparation of sample A by 5KDa ultrafiltration membrane filtration orthogonal test
[0028] After a single factor test on the factors affecting the membrane separation effect, it is determined that the influencing factors are rotational speed, time, pH value, concentration ratio, and finally determine th...
Embodiment 2
[0054] Example 2 : Preparation of Periplaneta americana ethanol extract by ultrafiltration membrane separation method to enrich effective fractions of polypeptides with a molecular weight less than 5KDa (sample C)
[0055] 2.1 Preparation of Periplaneta americana alcohol extract
[0056] Add 3 kg of Periplaneta americana medicinal materials, add 10,000 ml of 55% ethanol, soak and extract at room temperature, extract 3 times, each time for 24 hours. The extracts were combined, concentrated to dryness under reduced pressure, and dried in vacuum to obtain the alcoholic extract of Periplaneta americana.
[0057] 2.2 Preparation of peptide active substance (sample C) with molecular weight <5KDa
[0058] According to the extraction parameters optimized by the orthogonal test in Example 1, take 300 mg of the Periplaneta americana ethanol extract prepared in 2.1 and dissolve it in 1000 ml of warm water; In the ultrafiltration device of the ultrafiltration membrane, perform ultrafi...
Embodiment 3
[0063] Example 3 : The preparation of the acetone-water extract of Periplaneta americana to enrich the active parts of polypeptides with a molecular weight less than 5KDa by ultrafiltration membrane separation (sample D)
[0064] 3.1 Preparation of acetone-water extract of Periplaneta americana
[0065] Add 3 kg of Periplaneta americana medicinal materials, add 10,000 ml of 70% acetone aqueous solution, soak and extract at room temperature, extract 3 times, each time for 24 hours. The extracts were combined, concentrated to dryness under reduced pressure, and dried in vacuum to obtain the acetone-water extract of Periplaneta americana.
[0066] 3.2 Preparation of polypeptide sample (sample D) with molecular weight <5KDa
[0067] According to the extraction parameters optimized by the orthogonal test in Example 1, 300 mg of the Periplaneta americana acetone-water extract prepared in 2.1 was dissolved in 1000 milliliters of warm water; In an ultrafiltration device with a 5KD...
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