Preparation method for blattodea polypeptide substance, and medical use of blattodea polypeptide substance in anti-herpesvirus

A herpes simplex virus and cockroach technology, applied in the field of medicine, can solve the problems of inactive substances in the prevention and treatment of virus-infected diseases, such as literature reports, to achieve potential social and economic benefits, facilitate industrialization, and increase income

Inactive Publication Date: 2012-10-24
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of the novelty search show that there have been no previous reports on the preparation of pharmaceutical preparations prepared from the extract obtained from the ethanol extract of...

Method used

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  • Preparation method for blattodea polypeptide substance, and medical use of blattodea polypeptide substance in anti-herpesvirus
  • Preparation method for blattodea polypeptide substance, and medical use of blattodea polypeptide substance in anti-herpesvirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 : Preparation of Periplaneta americana aqueous extract enriched by ultrafiltration membrane separation method for effective fractions of polypeptides with a molecular weight less than 5KDa (sample A)

[0025] 1.1 Preparation of Periplaneta americana Water Extract

[0026] Add 3 kg of Periplaneta americana medicinal materials, add 10,000 ml of double distilled water, soak and extract once at 45°C for 24 hours; soak and extract once at 50°C for 24 hours; soak and extract once at 55°C for 24 hours. The extracts were combined, concentrated to dryness under reduced pressure, and dried in vacuum to obtain the aqueous extract of Periplaneta americana.

[0027] 1.2 Preparation of sample A by 5KDa ultrafiltration membrane filtration orthogonal test

[0028] After a single factor test on the factors affecting the membrane separation effect, it is determined that the influencing factors are rotational speed, time, pH value, concentration ratio, and finally determine th...

Embodiment 2

[0054] Example 2 : Preparation of Periplaneta americana ethanol extract by ultrafiltration membrane separation method to enrich effective fractions of polypeptides with a molecular weight less than 5KDa (sample C)

[0055] 2.1 Preparation of Periplaneta americana alcohol extract

[0056] Add 3 kg of Periplaneta americana medicinal materials, add 10,000 ml of 55% ethanol, soak and extract at room temperature, extract 3 times, each time for 24 hours. The extracts were combined, concentrated to dryness under reduced pressure, and dried in vacuum to obtain the alcoholic extract of Periplaneta americana.

[0057] 2.2 Preparation of peptide active substance (sample C) with molecular weight <5KDa

[0058] According to the extraction parameters optimized by the orthogonal test in Example 1, take 300 mg of the Periplaneta americana ethanol extract prepared in 2.1 and dissolve it in 1000 ml of warm water; In the ultrafiltration device of the ultrafiltration membrane, perform ultrafi...

Embodiment 3

[0063] Example 3 : The preparation of the acetone-water extract of Periplaneta americana to enrich the active parts of polypeptides with a molecular weight less than 5KDa by ultrafiltration membrane separation (sample D)

[0064] 3.1 Preparation of acetone-water extract of Periplaneta americana

[0065] Add 3 kg of Periplaneta americana medicinal materials, add 10,000 ml of 70% acetone aqueous solution, soak and extract at room temperature, extract 3 times, each time for 24 hours. The extracts were combined, concentrated to dryness under reduced pressure, and dried in vacuum to obtain the acetone-water extract of Periplaneta americana.

[0066] 3.2 Preparation of polypeptide sample (sample D) with molecular weight <5KDa

[0067] According to the extraction parameters optimized by the orthogonal test in Example 1, 300 mg of the Periplaneta americana acetone-water extract prepared in 2.1 was dissolved in 1000 milliliters of warm water; In an ultrafiltration device with a 5KD...

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Abstract

The present invention relates to a preparation method for a blattodea polypeptide substance, and a medical use of the blattodea polypeptide substance in anti-herpesvirus. Specifically the present invention relates to a blattodea extract effective fraction having a function of prevention and treatment of herpes simplex virus infection diseases, a preparation method and a medical use thereof. The Periplaneta Americana extract effective fraction of the present invention is a polypeptide substance with a molecular weight less than 5000 dalton, and is prepared by the following steps: carrying out extraction on fresh polypide or dried polypide of Periplaneta Americana with water or an organic solvent and a buffer, and then carrying out a membrane separation method to refine to obtain the product of the present invention, wherein the polypeptide active substance of the effective fraction has significant anti-herpes simplex virus (HSV-1 and HSV-2) activities, can be prepared into forms of a hydrogel, a cataplasm, lyophilized powder, a water agent, an aerosol, a suppository, a film agent, an external application liniment, and an ointment, and can be used for preparations of drugs for prevention and treatment of herpes simplex virus infections, daily chemical products or medical devices.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular, the invention relates to an effective part of a cockroach extract capable of preventing and treating herpes simplex virus infection, a preparation method and a medical application thereof. The effective part is a polypeptide active substance with a molecular weight of less than 5000 Daltons, which can be prepared into hydrogels, cataplasms, freeze-dried powders, waters, aerosols, suppositories, films, liniments for external use, and ointments etc., for the preparation of antiviral drugs, daily chemical products and / or medical devices. Background technique [0002] The diameter of herpes simplex virus is about 120-150 microns, the core containing DNA is located in the middle, and outwardly consists of three concentric structures of envelope, body coat, and capsid. The surface of the capsid is composed of 162 shell particles in a 3:3: The 2-axis symmetrical icosahedron can survive ...

Claims

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Application Information

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IPC IPC(8): A61K38/00A61K35/64A61P31/22
Inventor 戴志明杨荣新张成桂巫秀美赵昱
Owner DALI UNIV
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