PCR determination method for Chinese medicine or Chinese medicinal crops derived from eukaryote

Abstract

The invention provides a PCR identification method derived from eucaryote for Chinese traditional medicine and traditional Chinese medicinal materials. PCR augmentation is processed on DNA samples to obtain augmentation production according to a species-specific primer, wherein, the species-specific primer is designed according to a SINE sequence of eukaryotic genomes. The DNA samples are extracted from the Chinese traditional medicine and traditional Chinese medicinal materials, in particular from the further processing type Chinese traditional medicine. Species categories of eukaryotic species in the further processing type Chinese traditional medicine are estimated by analyzing the augmentation production, and the truth of the identified samples is further estimated. The PCR identification method is suitable for Chinese traditional medicine and traditional Chinese medicinal materials, is particularly suitable for the processing type Chinese traditional medicine, especially for the Chinese traditional medicine which is with extremely little DNA content and extremely short segment due to the further processing. The identification method has the advantages of simple operation, rapidness and sensitivity, low cost and effective truth identification of the Chinese traditional medicine and the traditional Chinese medicinal materials.

Description

technical field [0001] The present invention relates to the technical field of identification of traditional Chinese medicine and Chinese medicinal materials, more specifically, relates to the technical field of identification of traditional Chinese medicine and Chinese medicinal materials derived from eukaryotic organisms, especially refers to the PCR identification method of traditional Chinese medicine and Chinese medicinal materials derived from eukaryotic organisms, very It is suitable for deep processing of traditional Chinese medicine, especially the gelatinous traditional Chinese medicine refined from animal skin and bone, as well as the single medicine in the compound Chinese medicine, unearthed Chinese medicinal materials, etc. Sensitive and rapid identification of Chinese herbal medicines. Background technique [0002] my country's traditional Chinese medicine is extensive and profound, and there are many kinds of substances that can be used as Chinese medicine. O...

AI Technical Summary

Problems solved by technology

There are only a few thousand copies of rDNA and mtDNA in a single cell. When the DNA content of the sample is abundant and relatively complete, it is very simple and effective to use these sequences to construct detection methods. However, gelatinous substances are a kind of deep processing The product, its DNA damage is extremely serious, its content is extremely small, and its fragments are extremely short. For gelatinous traditional Chinese medicines that have severely damaged DNA due to deep processing and have extremely low content, it is difficult to establish a PCR method based on these two sequences. Clear bands are amplified from the gel-like traditional Chinese medicine DNA extract solution. The actual test results show that if primers are designed according to mtDNA, it is indeed difficult to amplify clear bands from the gel-like material DNA, and they are not suitable for Detection of Deeply Processed Traditional Chinese Medicine

Therefore, only relying on these two commonly used sequences in species analysis cannot meet the needs of colloid detection in practical applications.

Images