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Pederin discrimination method

A technology of paederin and paederus, which is applied in the field of identifying paederin by liquid chromatography-mass spectrometry, can solve problems such as difficulties in the study of paederin extracts, and achieve excellent sensitivity, Easy to operate, easy to study the effect

Inactive Publication Date: 2011-05-25
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no identification method for toxic paederin, which makes the clinical research of poisonous paederin extract very difficult.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment

[0026] 1. Sample processing

[0027] The toxic paederus extract was diluted 100 times with 50% HPLC grade methanol.

[0028] 2. Data acquisition

[0029] a) Acquisition of precursor ion peak data

[0030] The conditions of liquid chromatography are: chromatographic column is Shiseido C18 / 100×2.1mm / 5u, mobile phase is methanol-water with a volume ratio of 70:30 and a solution system containing 1mM ammonium formate, flow rate is 0.2ml·min -1 , Column temperature is 30℃, sample volume is 10μl; mass spectrometry conditions are: Turbo Ionspray ion source, triple quadrupole mass detector, spray gas 13L / min, curtain gas 12L / min, spray voltage 5KV, focusing voltage 400V, The inlet voltage is 10V, the outlet voltage is 10V, and the auxiliary heater temperature is 450°C; the mass spectrometer working mode is set to Q1 scan, and the scan mass range is 400~800amu. Scan the poisonous paederus extract diluent in negative ion mode and positive ion mode, respectively. Obtain 0~8min chromatograms sca...

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Abstract

The invention discloses a discrimination method of Paederus comprising a sample processing and data capturing process, wherein the Paederus extract product is diluted by 100 times using 50% of HPLC methanol and the data capturing process comprises the following steps: respectively scanning the Paederus extract product diluent in anion mode and cation mode using the liquid chromagraphy / mass spectrometry to acquire parent ion peak value; performing the collision induced dissociation and scanning using trace sampling pump post-column injection system to acquire daughter ion peak data. The discrimination method of Paederus uses liquid chromagraphy / mass spectrometry to obtain the features that: excellent sensitivity, accurate discrimination result, efficiency and reliability, and convenient operation and provides convenience for the clinical research on the Paederus extract product.

Description

technical field [0001] The invention relates to a method for identifying paederin, in particular to a method for identifying paederin by means of liquid chromatography-mass spectrometry. Background technique [0002] Poisonous paederus is a genus of Paederidae, with more than 250 known species, which are produced in all parts of the world except Antarctica. There are 21 species of poisonous paederus reported in China. Poisonous paederus mainly lives in wet farmland, vegetable fields, and weeds, and is a common small insect in ravines and farmland. Common poisonous paederus in my country include green winged paederus (also known as fusiform poisonous paederus, yellow-footed paederus), black-footed paederus, hairy paederus, towel-shaped paederus There are 7 kinds of paederus, paederus small red, paederus ant, and paederus tibetan. Among them, paederus blue-winged ant is the most widely distributed, covering all provinces and Taiwan and other regions. [0003] Eggs, larvae, pu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 万平李利珍赵梅君胡佳耀
Owner SHANGHAI NORMAL UNIVERSITY
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