Application of hyaluronic acid and its salt, crosslinked hyaluronic acid and its salt in preparing medicament for treating or preventing sea-soaking injury

Abstract

The invention relates to an application of a hyaluronic acid and a salt thereof as well as a cross-linked hyaluronic acid and a salt thereof in preparation of drugs for treating and preventing seawater immersion injuries, water injuries and complications thereof. The hyaluronic acid and the salt thereof and the cross-linked hyaluronic acid and the salt thereof, having proper swelling capacity, excellent sea water insulating effect, skin adhesion in water and blood hemostatic function, can form a protective layer on the wounds to realize rapid insulation of hemostasis from sea water and, therefore, can be used to prevent and treat seawater immersion injury caused by immersion of wound in seawater and complications thereof and to promote wound healing.

Description

technical field

[0001] The present invention relates to the application of hyaluronic acid and its salts, cross-linked hyaluronic acid and its salts in the preparation of medicines for emergency rescue wounds, in particular to the preparation of hyaluronic acid and its salts, cross-linked hyaluronic acid and its salts Application in drugs for the treatment or prevention of seafoam injury, water trauma and its complications. It belongs to the field of medicine. Background technique

[0002] In sudden accidents at sea, naval battles and landing battles, due to the special environment, it is often difficult for the wounded to receive timely surgical treatment within 6-8 hours of the "surgery golden time". The increase in the infection rate has brought great difficulties to the rescue work. The most critical and difficult point in the early treatment of seafoam wounds is: how to stop bleeding quickly and easily and isolate the wound from seawater.

[0003] Seawater is a speci...

Examples

Embodiment 1

[0041] Preparation of cross-linked hyaluronic acid

[0042] Hyaluronic acid (molecular weight is 2,000,000) 5g, make 1% solution, adjust pH to 8.5 with 0.1mol / L NaOH, fully stir. Place in a refrigerator at 4°C for 2 hours. Take it out and add 300ml of 1,2,7,8-epoxyoctane at 4°C while stirring, stir thoroughly for 30 minutes, and react at a constant temperature for 24 hours at 25°C, adjust the pH to neutral with HCl, and transfer the reaction product to dialysis The bag was dialyzed alternately with 0.1 mol / L NaCl solution and 25% ethanol solution for 60 hours, and the solution in the dialysis bag was concentrated under reduced pressure at 40°C to one-third of the original volume. After taking it out, place it in a vacuum drying oven at 40°C and dry to constant weight to obtain a cross-linked hyaluronic acid gel with a swelling rate of 400 times in seawater.

Embodiment 2

[0044] Preparation of cross-linked hyaluronic acid

[0045] Hyaluronic acid 5g (molecular weight is 2,000,000) is made into a 2% solution, adjusted to pH 5.5 with 0.1mol / L HCl, and fully stirred. Place in a refrigerator at 4°C for 2 hours. Take it out and add 300ml of 1,2,5,6-epoxyhexane at 4°C while stirring, stir thoroughly for 30 minutes, and react at constant temperature for 12 hours at 35°C, adjust the pH to neutral with NaOH, and transfer the reaction product to dialysis The bag was dialyzed alternately with 0.1 mol / L NaCl solution and 25% ethanol solution for 60 hours, and the solution in the dialysis bag was concentrated under reduced pressure at 40°C to one-third of the original volume. After taking it out, put it in a vacuum drying oven at 40°C and dry it to constant weight to obtain a cross-linked hyaluronic acid gel with a swelling rate of 360 times in seawater.

Embodiment 3

[0047] Preparation of cross-linked hyaluronic acid

[0048] Hyaluronic acid 5g (molecular weight is 2,000,000) is made into a 3% solution, adjusted to pH 4.0 with 0.1mol / L HCl, and fully stirred. Place in a refrigerator at 4°C for 2 hours. After taking it out, add 150ml of 1,4-butanediol glycidyl ether at 4°C while stirring, stir thoroughly for 30 minutes, and react at a constant temperature for 12 hours at 30°C, adjust the pH to neutral with NaOH, and transfer the reaction product to a dialysis bag , with 0.1mol / L NaCl solution and 25% ethanol solution alternately dialyzed for 60 hours, the solution in the dialysis bag was concentrated under reduced pressure at 40°C to one-third of the original volume. After taking it out, put it in a vacuum drying oven at 40°C and dry it to constant weight to obtain a cross-linked hyaluronic acid gel with a swelling rate of 600 times in seawater.

technical field

[0001] The present invention relates to the application of hyaluronic acid and its salts, cross-linked hyaluronic acid and its salts in the preparation of medicines for emergency rescue wounds, in particular to the preparation of hyaluronic acid and its salts, cross-linked hyaluronic acid and its salts Application in drugs for the treatment or prevention of seafoam injury, water trauma and its complications. It belongs to the field of medicine. Background technique

[0002] In sudden accidents at sea, naval battles and landing battles, due to the special environment, it is often difficult for the wounded to receive timely surgical treatment within 6-8 hours of the "surgery golden time". The increase in the infection rate has brought great difficulties to the rescue work. The most critical and difficult point in the early treatment of seafoam wounds is: how to stop bleeding quickly and easily and isolate the wound from seawater.

[0003] Seawater is a speci...

Examples

Embodiment 1

[0041] Preparation of cross-linked hyaluronic acid

[0042] Hyaluronic acid (molecular weight is 2,000,000) 5g, make 1% solution, adjust pH to 8.5 with 0.1mol / L NaOH, fully stir. Place in a refrigerator at 4°C for 2 hours. Take it out and add 300ml of 1,2,7,8-epoxyoctane at 4°C while stirring, stir thoroughly for 30 minutes, and react at a constant temperature for 24 hours at 25°C, adjust the pH to neutral with HCl, and transfer the reaction product to dialysis The bag was dialyzed alternately with 0.1 mol / L NaCl solution and 25% ethanol solution for 60 hours, and the solution in the dialysis bag was concentrated under reduced pressure at 40°C to one-third of the original volume. After taking it out, place it in a vacuum drying oven at 40°C and dry to constant weight to obtain a cross-linked hyaluronic acid gel with a swelling rate of 400 times in seawater.

Embodiment 2

[0044] Preparation of cross-linked hyaluronic acid

[0045] Hyaluronic acid 5g (molecular weight is 2,000,000) is made into a 2% solution, adjusted to pH 5.5 with 0.1mol / L HCl, and fully stirred. Place in a refrigerator at 4°C for 2 hours. Take it out and add 300ml of 1,2,5,6-epoxyhexane at 4°C while stirring, stir thoroughly for 30 minutes, and react at constant temperature for 12 hours at 35°C, adjust the pH to neutral with NaOH, and transfer the reaction product to dialysis The bag was dialyzed alternately with 0.1 mol / L NaCl solution and 25% ethanol solution for 60 hours, and the solution in the dialysis bag was concentrated under reduced pressure at 40°C to one-third of the original volume. After taking it out, put it in a vacuum drying oven at 40°C and dry it to constant weight to obtain a cross-linked hyaluronic acid gel with a swelling rate of 360 times in seawater.

Embodiment 3

[0047] Preparation of cross-linked hyaluronic acid

[0048] Hyaluronic acid 5g (molecular weight is 2,000,000) is made into a 3% solution, adjusted to pH 4.0 with 0.1mol / L HCl, and fully stirred. Place in a refrigerator at 4°C for 2 hours. After taking it out, add 150ml of 1,4-butanediol glycidyl ether at 4°C while stirring, stir thoroughly for 30 minutes, and react at a constant temperature for 12 hours at 30°C, adjust the pH to neutral with NaOH, and transfer the reaction product to a dialysis bag , with 0.1mol / L NaCl solution and 25% ethanol solution alternately dialyzed for 60 hours, the solution in the dialysis bag was concentrated under reduced pressure at 40°C to one-third of the original volume. After taking it out, put it in a vacuum drying oven at 40°C and dry it to constant weight to obtain a cross-linked hyaluronic acid gel with a swelling rate of 600 times in seawater.

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