In vitro cultivation method Olfactory stem cell

An in vitro culture, stem cell technology, applied in embryonic cells, germ cells, animal cells, etc., can solve problems such as immaturity in clinical treatment of transplantation

Inactive Publication Date: 2009-05-13
BEIJING HONGTIANJI NEUROSCI ACADEMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, there are few reports on the biological characteristics and in vitro establishment and culture of olfactory mucosal neural stem cells, and the clinical application of human embryonic olfactory stem cells in the transplantation of central nervous system injuries is still immature

Method used

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Examples

Experimental program
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Embodiment Construction

[0006] 1. Collect fetuses without obvious developmental disorders from abortion and induced labor in obstetrics and gynecology department.

[0007] 2. Reagents DMEM / F12 (Gibco), B27 (Gibco), EGF (PEPRO TECH) FGF (PEPROTECH) L-Glutamine (Sigma) Collagenase (Sigma).

[0008] 3. Make a craniotomy along the upper brow arch of the dead fetus, remove the brain tissue, expose the anterior skull base, remove the olfactory bulbs to see the cribriform plate and cockscomb, cut the skull base bone plate around the cribriform plate, and cut the lateral walls of the nasal cavity on both sides in the direction of the nasal cavity The upper and middle turbinates and the corresponding middle and upper parts of the nasal septum were removed and washed several times in DMEM / F12 to wash away the blood on the olfactory mucosa. Transfer the crushed tissue into a 15ml centrifuge tube, add 5ml of basal medium, blow it up and down with a thick elbow pipette to make it a tissue suspension, centrifuge f...

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Abstract

The invention discloses an in vitro culture method for human embryonic olfactory mucosa neural stem cells. Adequate olfactory neural stem cells, olfactory neural precursor cells and olfactory ensheathing precursor cells are obtained by establishing the in vitro culture for the human embryonic olfactory mucosa neural stem cells, and are applied to the clinical treatment and fundamental research of cellular transplantation of central lesion.

Description

Technical field [0001] The patent of the present invention relates to an in vitro culture method for establishing human embryonic olfactory mucosal neural stem cells, so as to obtain a sufficient number of olfactory neural stem cells, olfactory neural precursor cells and olfactory sheath precursor cells for the clinical treatment of central nervous system injury by cell transplantation and basic research. Background technique [0002] At present, there are few reports on the biological characteristics and in vitro establishment and culture of olfactory mucosal neural stem cells, and the application of human embryonic olfactory stem cells in the clinical treatment of central nervous system injury is still immature. Contents of the invention [0003] In order to make it possible for human embryonic olfactory stem cells to be used in the clinical treatment of central nervous system injury transplantation, the patent of the present invention proposes an in vitro culture method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08A61K35/12A61P25/00A61P9/00A61K35/30C12N5/0735C12N5/0797
Inventor 黄红云
Owner BEIJING HONGTIANJI NEUROSCI ACADEMY
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