Cornea mid-term preservation liquid

Abstract

The invention relates to a cornea mid-term preservation liquid. 1000ml of preservation liquid contains 0.5-1.5g of DMEM culture medium, 1-10g of hydroxypropyl methylcellulose, 0.1-10ml of 2-mercaptoethanol, 10-20g of dextran (with the molecular weight of 40000), 1-3ml of nonessential amino acid, 10-32mmol of Hepes buffer solution and 0.01-0.05mmol of dexamethasone, 50-70mg of gentamicin. The pH value of the preservation liquid is 7.2-8.0, and the permeation pressure is 350-400mmol / LH2O. The cornea mid-term preservation liquid not only has good cornea storage quality, but also has the advantage of good cost performance and is the cornea mid-term preservation liquid which is widely applicable to the eye bank at home and abroad.

Description

technical field [0001] The invention relates to a mid-term corneal preservation solution. Background technique [0002] The existing active preservation methods of cornea can be divided into short-term preservation, medium-term preservation and long-term preservation according to the difference of preservation time. For short-term preservation, the whole eyeball is sterilized and then stored in a wet room at 4°C. The advantage of this method is that it is simple, effective and feasible, and is especially suitable for corneal transplantation in primary hospitals. The disadvantage is that the storage time is short, and it is generally valid within 48 hours. Sometimes the patient cannot be found in time and the materials have expired and are discarded. For long-term preservation, the corneal slices are specially treated and stored in liquid nitrogen at -196°C. The biggest advantage of cryopreservation is to provide high-quality active cornea for clinical use at any time, but ...

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