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Anti-idiotype conjugate and its use as a standard in an immunassay

An anti-idiotype antibody, conjugate technology, applied in specific peptides, immunoglobulins, antibody mimics/scaffolds, etc.

Active Publication Date: 2009-09-23
F HOFFMANN LA ROCHE & CO AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to Mire-Sluis et al., well known formats of anti-drug antibody assays show significant disadvantages

Method used

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  • Anti-idiotype conjugate and its use as a standard in an immunassay
  • Anti-idiotype conjugate and its use as a standard in an immunassay
  • Anti-idiotype conjugate and its use as a standard in an immunassay

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Embodiment Construction

[0023] The invention includes conjugates comprising an anti-idiotype antibody that specifically binds to a CDR region of a parent antibody, and a reference immunoglobulin belonging to a single immunoglobulin class. Preferably the parent antibody is a therapeutic antibody. Preferably said parent antibody is a diagnostic antibody.

[0024] The term "anti-idiotypic antibody specifically binding to the CDR region of the parent antibody" in the present invention means an antibody raised against the parent antibody in a non-human animal, that is, a non-human antibody. Preferably, the antibody produced against the parental antibody is produced in rodents or macaques, particularly preferably in mice, rabbits or cynomolgus monkeys, or obtained by display technology, preferably phage or ribosome display technology. Preferably said anti-idiotypic antibody is a polyclonal antibody. It is also preferred that the anti-idiotypic antibody is a monoclonal antibody. The animal is preferably ...

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PUM

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Abstract

A composition comprising a conjugate of an anti-idiotype antibody specifically binding to a CDR region of a parent antibody and method of using polyclonal human serum immunoglobulin of class E, G, M, or A, and the use of said composition as a standard in an immunoassay is presented.

Description

technical field [0001] The invention includes the conjugates, and their use as standards or positive controls in immunoassays for measuring anti-drug antibodies. Background technique [0002] Standard solid-phase immunoassays using monoclonal antibodies involve the separation between an antibody adsorbed on a solid support (capture antibody), an antigen, and an antibody directed against another epitope of the antigen conjugated to a detectable label (tracer antibody). form a complex. Thus a sandwich layer is formed: solid phase support - capture antibody - antigen - tracer antibody. In the sandwich, the intensity of the antibody-conjugated detectable label is proportional to the antigen concentration in the incubation medium. Since the capture antibody and the tracer antibody bind to different epitopes of the antigen, the standard sandwich method is also called double antigen bridging immunoassay (double antigen bridging immunoassay). Hoesel, W. et al. in J. Immunol. Meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/42G01N33/541
CPCG01N2333/435G01N2021/5903C07K2316/52C07K2319/30G01N33/553C07K16/461G01N33/531C07K16/4291C07K2317/54C07K16/4283G01N33/543C07K2317/31C07K16/4208G01N2800/52C07K2317/52G01N33/6857G01N2800/24G01N33/686C07K2317/24C07K16/4258C07K16/42G01N33/541
Inventor K-G·施图本拉赫R·福格尔U·韦塞尔斯
Owner F HOFFMANN LA ROCHE & CO AG
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