A kind of preparation method of oligomer grape seed proanthocyanidin

A proanthocyanidin and grape seed technology, applied in the direction of organic chemistry, can solve the problems of low oligomer content, complex process, high production cost, etc.

Active Publication Date: 2011-12-28
QINGDAO UNIV +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a new method for separating and purifying oligomer grape seed proanthocyanidins with ultrafiltration membrane and nanofiltration membrane in order to overcome the defects of low oligomer content, complex process and high production cost in the products existing in the current proanthocyanidin extraction method. method, the content of oligomer components can be as high as 60%, which is about double that of similar products at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0011] 1. Take 100 grams of grape seeds, rinse them with clean water, add 200ml of 35% ethanol solution for extraction for 20 hours, remove the grape seeds by filtration, and collect the extract.

[0012] 2. Adjust the pH value of the above extract to 8.5 with NaOH solution, stir gently for 3 minutes, and let it stand for 6 hours to precipitate a brick-red proanthocyanidin coarse precipitate, filter and collect the precipitate, about 1.5 grams. The precipitate was dissolved in 15 ml of 35% ethanol solution with a pH value of 4.0, the undissolved non-procyanidin precipitate was removed by suction filtration, and the filtrate containing dissolved proanthocyanidin was collected.

[0013] 3. Filter the above-mentioned filtrate with an ultrafiltration membrane with a pore size of 0.001 μm and a pressure of 0.25 MPa to remove the precipitate, and then filter the filtrate with a nanofiltration membrane with a pore size of 0.0001 μm under a pressure of 1.0 Mpa to collect the retained p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A method for preparing oligomeric grape seed proanthocyanidins. After the grape seeds are rinsed with clean water, they are added to an ethanol solution for leaching, the grape seeds are removed by filtration, and an extract is collected; Brick red procyanidin crude precipitate, suction filtration and collect the precipitate; dissolve the precipitate in ethanol solution, collect the filtrate containing dissolved procyanidin; then filter the filtrate with nanofiltration membrane to collect the oligomers that are trapped and precipitated on the filter membrane The oligomeric proanthocyanidins are obtained by washing the precipitates with acetone and ethanol respectively, and drying them in a drying oven.

Description

Technical field: [0001] The invention relates to a preparation method of oligomer grape seed proanthocyanidins. The oligomer proanthocyanidins are prepared by ethanol solution extraction, acid-base condition purification, and nanofiltration membrane separation, and belong to the field of extraction of natural active substances in biotechnology. Background technique: [0002] Proanthocyanidins, often called procyanidine or proanthocyanidins abroad, are natural phytochemicals formed by the polymerization of different amounts of catechin or epicatechin monomers. They are widely found in various plants such as oranges, lemons, olives, and grapes. Among them, it has good anti-oxidation and free radical scavenging effects, and has many applications in food, health products, pharmaceuticals and other fields. Among them, grape seeds are easy to preserve and process, and are rich in proanthocyanidins, so in the prior art, grape seeds are mostly used as raw materials to extract proant...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62
Inventor 钟进义王可平
Owner QINGDAO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap