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Double-functional fusion protein based on antibacterial peptide, preparation method and applicaitoin thereof

A fusion protein and dual-function technology, applied in the field of biomedicine, to achieve the effect of promoting rapid healing, simple and easy purification method, and prolonging half-life

Inactive Publication Date: 2012-05-23
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no human epidermal growth factor with prokaryotic expression on the market for clinical treatment

Method used

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  • Double-functional fusion protein based on antibacterial peptide, preparation method and applicaitoin thereof
  • Double-functional fusion protein based on antibacterial peptide, preparation method and applicaitoin thereof
  • Double-functional fusion protein based on antibacterial peptide, preparation method and applicaitoin thereof

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Experimental program
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Embodiment Construction

[0047] The antimicrobial peptide-based bifunctional fusion protein provided by the present invention is a fusion protein based on cecropin B protein sequence and human epidermal growth factor, and its connecting protein is specially designed as a site that thrombin can recognize, and on the fusion protein Construct a purified His-tag.

[0048] The present invention adopts the prokaryotic expression system for the above fusion protein to realize its biological preparation, and obtains the purified fusion protein.

[0049] The purified fusion protein was used to carry out antibacterial tests and live animal verification, indicating that the fusion protein can be applied to the preparation of drugs for the treatment of epidermal wounds, for wounds, abrasions, burns, scalds and other diseases of the epidermis, and can prevent wounds Infect.

[0050] The present invention will be described in further detail in the following knot and accompanying drawings, which are explanations of...

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Abstract

The invention discloses a double-functional fusion protein based on an antibacterial peptide, a preparation method and an application thereof. The double-functional fusion protein based on the antibacterial peptide is a fusion protein based on a cecropins B protein sequence and a human epidermal growth factor; the linking protein is especially designed to be a locus which can be identified by thrombin; and a purified His tag is constructed on the fusion protein. In the invention, the biological preparation for the fusion protein is realized by using a prokaryotic expression system and the purified fusion protein is obtained. A bacteriostasis test and a verification for realizing live animals are performed by the purified fusion protein to indicate that the fusion protein can be applied topreparing a medicine for treating epidermal wound, can be applied to diseases such as epidermal wound, abrade, burn and scald, etc., and can prevent wound from being infected.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the artificial design and synthesis of biologically active polypeptides, in particular to a bifunctional fusion protein based on antibacterial peptides and its preparation method and application. Background technique [0002] 1. Research progress of antimicrobial peptides [0003] With the widespread use of "traditional antibiotics", a large number of drug-resistant strains have been produced, and when pathogenic bacteria are infected, if antibiotics are used for treatment, it will stimulate the release of endotoxins in the body and cause damage to the body. Therefore, searching for a new generation of antibacterial agents has become a hot spot in the development of new drugs, and the discovery of antimicrobial peptides overcomes the above shortcomings of traditional antibiotics. [0004] Antimicrobial peptide is a general term for a class of short peptides with antibacterial activity. I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/70C12P21/02A61K38/18A61P17/02C12R1/19
Inventor 万一韩丽萍沈卫荣张月娟孙晓宇张琨李玥
Owner MICROBIOLOGY INST OF SHAANXI
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