Method for manufacturing aptamer biosensor used for quickly detecting antibiotic and heart disease markers

A biosensor and a manufacturing method technology, applied in the field of biodetection, can solve the problem of no biosensor, etc., and achieve the effects of low cost, high specificity, and increased reliability

Inactive Publication Date: 2010-06-16

TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY

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Problems solved by technology

[0003] According to the search, there are currently no aptamer biosensors for t...

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Abstract

The invention relates to a method for manufacturing an aptamer biosensor used for quickly detecting antibiotic and heart disease markers, which comprises the following steps: 1, modifying an electrode; and 2, fixing an aptamer. In the aptamer biosensor of the invention, an antibiotic and heart disease marker (cardiac troponin I) aptamer serving as a recognition molecule is fixed on the surface ofa glassy carbon electrode to specifically combine and quickly detect an antibiotic or an cardiac troponin I (cTnI) in a specimen, and an electrochemical signal generated can reflect the concentrationof the antibiotic or cTnI in solution of the specimen. The biosensor has the advantages of high specificity, easy carrying, quickness, high stability, low cost and the like.

Application Domain

Material electrochemical variables

Technology Topic

Glassy carbon electrodeRapid detection +8

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Examples

Effect test(1)

Test Example

[0023] Application examples of the aptamer biosensor:

[0024] 1. Sample determination: with different concentrations of tetracycline, oxytetracycline, penicillin, cTnI solution, every 5 minutes, do cyclic voltammetry test in the potential range of -0.1-0.6, the scanning rate is 0.05V/s.

[0025] 2 Results and discussion: The response range of the aptamer to the detection of tetracycline, oxytetracycline, penicillin and cTnI.

[0026] Under the selected optimized conditions, use the modified working electrode with a series of different concentrations (0mg/L, 0.001mg/L, 0.005mg/L, 0.01mg/L, 0.015mg/L, 0.02mg/L) , 0.03mg/L, 0.05mg/L, 0.08mg/L, 0.1mg/L) tetracycline/oxytetracycline/penicillin/cTnI potassium ferricyanide PBS solution or milk solution reaction, determine the cyclic voltammetry curve, and do Draw a standard curve of concentration versus spike current (e.g. figure 1 with figure 2 ). The concentration of tetracycline, oxytetracycline, penicillin or cTnI in the specimen can be detected by the same method and calculated from the standard curve.

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Description & Claims & Application Information

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Classification and recommendation of technical efficacy words

Good stability
High specificity

Method for manufacturing aptamer biosensor used for quickly detecting antibiotic and heart disease markers

A biosensor and a manufacturing method technology, applied in the field of biodetection, can solve the problem of no biosensor, etc., and achieve the effects of low cost, high specificity, and increased reliability

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Image

Examples

Test Example

PUM

Description & Claims & Application Information

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