Method for manufacturing aptamer biosensor used for quickly detecting antibiotic and heart disease markers
A biosensor and a manufacturing method technology, applied in the field of biodetection, can solve the problem of no biosensor, etc., and achieve the effects of low cost, high specificity, and increased reliability
Inactive Publication Date: 2010-06-16
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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[0003] According to the search, there are currently no aptamer biosensors for t
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The invention relates to a method for manufacturing an aptamer biosensor used for quickly detecting antibiotic and heart disease markers, which comprises the following steps: 1, modifying an electrode; and 2, fixing an aptamer. In the aptamer biosensor of the invention, an antibiotic and heart disease marker (cardiac troponin I) aptamer serving as a recognition molecule is fixed on the surface ofa glassy carbon electrode to specifically combine and quickly detect an antibiotic or an cardiac troponin I (cTnI) in a specimen, and an electrochemical signal generated can reflect the concentrationof the antibiotic or cTnI in solution of the specimen. The biosensor has the advantages of high specificity, easy carrying, quickness, high stability, low cost and the like.
Description
technical field [0001] The invention belongs to the technical field of biological detection, in particular to a manufacturing method of an aptamer biosensor for rapid detection of antibiotics and heart disease markers. Background technique [0002] Aptamers have the advantages of wide target molecules, strong specificity, and stability, and can be applied to biosensors, and can solve some shortcomings in sensor detection. Aptamers as recognition molecules have been successfully applied to various biosensor platforms, showing Good application prospects. [0003] According to the search, there is no aptamer biosensor for the detection of tetracycline, oxytetracycline, penicillin and cTnI in the market at present. Contents of the invention [0004] The purpose of the present invention is to overcome the disadvantages of the prior art, and provide a method for making an aptamer biosensor for rapid detection of antibiotics and heart disease markers, which has a simple preparat...
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Inventor 张娟琨吴艳范婷张彬彬
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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