Preparation method of epimedium aglycone

Abstract

The invention relates to a preparation method of epimedium aglycone, which comprises the steps that dried epimedium is pulverized or cut into segments, 30-95% ethanol or 30-100% methanol is added for reflux extraction, the extract solution is decompressed and concentrated to obtain paste, 10-100 ml of acetate-sodium acetate buffer solution with the pH of 3.5-9.0 is added into each gram of paste, snailase is added for hydrolysis, the mass ratio of enzyme to paste is 0.05-5, the reaction temperature is 15 to 80 DEG C, the reaction time is 2 to 72h, the reaction solution is centrifuged, the precipitate is dissolved with an organic solvent, filtration is carried out to obtain filtrate, the solvent is steamed away to obtain crude epimedium aglycone, and pure epimedium aglycone is obtained after recrystallization. The invention has the characteristic that the epimedium extract is directly converted into high-purity aglycone in one step by utilizing the strong enzymic hydrolysis of the snailase so as to simplify the operation steps with strong purposiveness and high conversion rate, and has the advantages of no pollution and large-scale production. The invention overcomes the shortcomings of large destruction on the aglycone, serious pollution, poor purposiveness, low conversion rate, difficult separation and purification and the like of the prior art.

Description

Technical field: [0001] The invention belongs to the field of natural medicines and relates to a preparation method of icariin. Background technique: [0002] Epimedium was first recorded in "Shen Nong's Materia Medica", which has the functions of nourishing kidney and strengthening yang, dispelling wind and dampness, strengthening muscles and strengthening bones, etc. It is clinically used to treat osteoporosis caused by various reasons. Experiments have shown that the flavonoid components of Epimedium are the effective parts of its anti-osteoporosis, and the active ingredient icarigenin has the most obvious effect, which can promote the activity of osteoblasts and inhibit the activity of osteoclasts in vitro. Therefore, the preparation of Epimedium Amarigenin is of great value in the development of new anti-osteoporosis drugs. [0003] Icaritin, full name 3,5,7-trihydroxy-4′-methoxy-8-prenyl flavone, also known as icaritin, English name Icaritin, is a class of polyhydroxy...

AI Technical Summary

Problems solved by technology

(2) Biotransformation method, such as using Epimedium as the fermented enzyme inducer of bacteria, molds, and yeasts to prepare a mold-containing liquid, and then use this bacterial liquid to convert Epimedium flavonoid glycosides into low glycosides or glycosides Yuan (publication number: CN1473938A enzymatically hydrolyzing icariin glycosyl to prepare low-sugar icariin or aglycone), because there are many kinds of epimedium flavonoid glycosides, and its glycosidic bond structure is very complicated, mainly composed of α- L-rhamnosidic bonds, β-D-glucosidic bonds, β-D-xyloglycosidic bonds and other monosaccharide glycosidic bonds and disaccharide glycosidic bonds composed of them, plus the high specificity of enzyme-catalyzed hydrolysis , these enzyme-containing bacterial solutions cannot completely remove the glycosidic bonds, so it is impossible to obtain icariin with higher purity

There is no report on the preparation of icariin by using helicase

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