Device for culturing endothelial cells of myocardial bridge-mural coronary artery

Abstract

The invention relates to a device for culturing endothelial cells of myocardial bridge-mural coronary artery, which is characterized in that a pair of myocardial bridge press block and simulated coronary artery is arranged in an airtight cavity, a motor drives the myocardial bridge press block to press the simulated coronary artery, and the outer part of the airtight cavity is connected with a proportional pressure-regulating constant-current stabilizer consisting of an air compressor, an air reservoir, a proportional pressure valve and a tank pressure sensor; after a periodic pulsatile flow produced by a heart pump passes through an energy accumulator, the liquid is divided into two branches, i.e. one branch of the fluid flows through a division to flow into a liquid storage tank and a constant temperature device after loading, and the other branch of the fluid flows through the airtight cavity to flow into the liquid storage tank and the constant temperature device after loading. Inthe invention, the endothelial cells are cultured in the pulsatile flow, so that the physiological environment of the coronary artery periodically oppressed by the myocardial bridge can be simulated,and the stress on the endothelial cells in the coronary artery can be independently adjusted relatively, therefore, the method of the invention provides an experimental means for studying the relation between the form and function of the endothelial cell and the proximal coronary atherosclerotic diseases in the presence of the myocardial bridge.

Description

technical field [0001] The invention relates to a culture device for vascular endothelial cells, in particular to a culture device for simulating that endothelial cells are periodically compressed by myocardial bridges in coronary arteries and under the synergistic effects of circumferential stress, shear stress and normal stress. Background technique [0002] Myocardial bridge is a relatively common anatomical phenomenon. Due to the influence of myocardial bridge, atherosclerosis is more likely to occur in the proximal part of the coronary artery, but atherosclerosis is rare in the lower part of the bridge and the distal part. The morphology and function of vascular endothelial cells are closely related to the formation of atherosclerosis. Nowadays, the method of endothelial cell culture to study the formation mechanism of proximal coronary atherosclerosis has been widely used, and the types and methods of endothelial cell culture devices are also increasing. [0003] The ...

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Problems solved by technology

[0003] The endothelial cells in the static culture method are not subjected to the shear stress of the blood flow, and the longer the in vitro time, the poorer the differentiation ability, and it is difficult to tolerate the hemodynamic environment in subsequent experiments, and the cells fall off

Parallel plate flow chambers and strain chambers can be used to study the effects of flow shear stress and circumferential stress on endothelial cells, respectively, but neither of these two devices can simulate the stress on endothelial cells in coronary arteries in the presence of myocardial bridges

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