Melamine hapten, melamine complete antigen and preparation method thereof

A technology of melamine and complete antigen, applied in chemical instruments and methods, animal/human protein, serum albumin, etc., can solve problems such as high nitrogen content, falsely high protein content, people's health and life hazards, and achieve high sensitivity , good effect of specificity

Inactive Publication Date: 2011-01-26
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because of its high nitrogen content, adding it to food can produce the false appearance of high protein content, so it was illegally added to milk and other protein products, which led to the "Sanlu milk powder

Method used

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  • Melamine hapten, melamine complete antigen and preparation method thereof
  • Melamine hapten, melamine complete antigen and preparation method thereof
  • Melamine hapten, melamine complete antigen and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

Example 1. Preparation and identification of melamine hapten

1. Preparation of melamine hapten

1. Weigh 300mg of 2-chloro-4,6-diamino-1,3,5-triazine (purchased from Sigma, CAS number: 3397-62-4), 340μL 3-mercaptopropionic acid, 168mg hydrogen carbonate Place the sodium in a 50mL flask, then add 30mL of a 50% volume concentration of ethanol aqueous solution, heat to reflux at 100°C under nitrogen protection, and continue to stir for 10 hours;

2. The product of step (1) is evaporated to dryness under reduced pressure, and 50mL is used with a mass concentration of 5% NaHC0 3 Dissolve in the aqueous solution, then adjust the pH of the solution to 2 with 1mol / L HCl aqueous solution;

3. Extract the product of step (2) with chloroform for 3 times, discard the organic phase and leave the aqueous phase, and adjust the pH of the aqueous phase to 3 with concentrated KOH, and a white precipitate appears;

4. The precipitate was collected by filtration and dried naturally to obtain 260 mg ...

Example Embodiment

The melamine complete antigen of the present invention is a conjugate of a compound represented by formula II and a carrier protein.

(1) The molar ratio is 1:3:2. Take 2-chloro-4,6-diamine-1,3,5-triazine, 3-mercaptopropionic acid and potassium hydroxide as raw materials, press 13mg: Dissolve the raw materials in an ethanol aqueous solution with a volume concentration of 40% in a ratio of 1 ml, heat to reflux, and under nitrogen protection, and continue to stir for 15 hours;

Example Embodiment

The third object of the present invention is to provide a complete melamine antigen.

(1) The molar ratio is 1:1:1. Take 2-chloro-4,6-diamino-1,3,5-triazine, 3-mercaptopropionic acid and sodium hydroxide as raw materials, press 17mg: Dissolve the raw materials in an ethanol aqueous solution with a volume concentration of 60% in a ratio of 1 ml, heat to reflux, and under nitrogen protection, and continue to stir for 8 hours;

(2) Spin dry the product of step (1) and add a 10% aqueous sodium bicarbonate solution to dissolve, and adjust the pH to 1;

(3) Extract the product of step (2) with chloroform, discard the organic phase, adjust the pH of the aqueous phase to 3, and obtain a white precipitate with a yield of 86%. It is identified as the melamine hapten represented by formula II, and its chemical name is: 2-(2-carboxyethyl)thio-4,6-diamino-1,3,5-triazine.

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PUM

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Abstract

The invention discloses a melamine hapten, a melamine complete antigen and a preparation method thereof, wherein, the melamine complete antigen has a structure as follows: the complete antigen can maintain better physicochemical properties of the original compound such as a chemical structure, charge distribution, a space structure and the like and obtain an antibody with good specificity and high sensitivity while immunizing an experimental animal, thus laying a foundation for subsequent establishment of a rapid, sensitive and efficient ELISA melamine detection method.

Description

technical field The invention relates to a melamine hapten, a melamine complete antigen, a preparation method of the melamine hapten and a preparation method of the melamine complete antigen. Background technique The people take food as their heaven and food safety as their first priority, and food safety is related to the health and safety of the people. However, the Sanlu milk powder incident in 2008 caused 300,000 children in China to suffer from diseases, and at least 6 babies died, which broke the hearts of countless families. After the incident, China issued a notice on the provisional management limit value of melamine in dairy products and foods containing milk. However, incidents of illegally adding melamine have been repeatedly banned. Afterwards, it was reported that in December 2009, Shanghai Panda and Shaanxi Jinqiao Dairy milk powder was found to have exceeded the standard of melamine; Lekang Dairy melamine case was detected and melamine-exceeding milk powder...

Claims

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Application Information

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IPC IPC(8): C07D251/52C07K14/765C07K14/77C07K14/795
Inventor 高志贤凌华浓周青青陈超柳明宁保安
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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