Rapid test card of glibenclamide and test method of glibenclamide

A technology of glibenclamide and detection card, which is applied in the field of detection of illegally added western medicine ingredients in traditional Chinese medicine, can solve problems such as cumbersome operation, and achieve the effects of high sensitivity, easy preparation, and rapid detection

Inactive Publication Date: 2011-04-13
无锡安迪生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of detection instrument that can detect glibenclamide, directly apply the method of immunology to detect the content of glibenclamide in the hypoglycemic traditional Chinese medicine sample, overcome the disadvantages such as the tedious operation of existing chromatographic detection, establish Specificity-based detection methods based on immunology

Method used

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  • Rapid test card of glibenclamide and test method of glibenclamide
  • Rapid test card of glibenclamide and test method of glibenclamide
  • Rapid test card of glibenclamide and test method of glibenclamide

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Effect test

Embodiment 1

[0023] A quick test card for glibenclamide, which is provided with a test strip 2 in the test card shell 1, the structure of the test card shell 1 and the setting of the test strip 2 refer to the attached figure 1 , The detection card shell 1 is a long flat thin shell shell, 70mm long, 20mm wide, 5mm thick, and the thin shell wall thickness is 1mm, made of engineering plastics. The detection card casing 1 is composed of two halves of a casing cover and a casing seat, and a detection window 3 and a sample injection hole 4 are arranged on the casing cover of the detection card casing 1 . The test strip 2 is placed in the shell seat of the test card shell 1 .

[0024] The test strip 2 is a thin strip with a multi-layer structure, the length of the strip is about 60mm, the width of the strip is about 4mm, and the thickness is about 2.5mm. The test strip 2 has a multi-layer structure: the bottom layer is a supporting backboard 5, which is a polyvinyl chloride plastic sheet with a ...

Embodiment 2

[0027] When using the glibenclamide rapid detection card for detection, the sample liquid to be detected is dripped from the sample injection hole 4 onto the sample pad 8 of the glibenclamide rapid detection card, and due to the principle of siphon action, the sample liquid to be detected is driven The sample solution and the colloidal gold marker of the anti-glibenclamide monoclonal antibody contained in the colloidal gold membrane 7 diffuse to the nitrocellulose membrane 6 together, and the results are observed within 5-10 minutes.

[0028] Immunological methods are used to directly detect the content of glibenclamide in the samples. The samples to be tested are capsules, tablets and pills of hypoglycemic traditional Chinese medicine samples. The processing methods are as follows:

[0029] (1) Randomly collect a certain amount of samples to be tested and crush them evenly;

[0030] (2) Weigh 5g of the pulverized sample, add 10mL of sample extract, stir and mix for more than ...

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Abstract

The invention discloses a rapid test card of glibenclamide and a test method of the glibenclamide, which belong to the technical field of testing western medicament components illegally added in Chinese patent medicaments. A test strip is arranged in an outer shell of the rapid test card of the glibenclamide, and a sample pad, a collaurum membrane, a nitrocellulose membrane and a water absorbent membrane are adhered to a support back plate in turn, wherein the collaurum membrane is a glass fiber membrane containing an anti-glibenclamide monoclonal antibody collaurum marker; and two detection strips are arranged on the nitrocellulose membrane, one of the detection strips contains glibenclamide protein conjugate, and the other one of the detection strips contains anti-rabbit antibody or anti-mouse antibody. The rapid test card of the glibenclamide has the advantage that the content of the glibenclamide illegally added in a hypoglycemic Chinese medicament sample can be detected directly by using an immunological method. The rapid test card of the glibenclamide is easy to prepare, is convenient and rapid to use, and generates accurate results.

Description

technical field [0001] The invention relates to a glibenclamide rapid detection card and a detection method thereof, is a detection device for detecting glibenclamide, and belongs to the technical field of detection of illegally added western medicine components in traditional Chinese medicine. Background technique [0002] It is legal to add western medicine ingredients to Chinese patent medicines. For example, glibenclamide is added to Xiaoke Pills, which significantly improves the curative effect. The other type is the illegal addition of western medicine. The instructions do not specify what kind of western medicine is added to it. This is the most dangerous, and improper use by patients will inevitably lead to catastrophe. [0003] The most commonly added hypoglycemic western medicine ingredients in diabetes medicines or health care products are glibenclamide, gliclazide and glipizide. Glibenclamide, also known as glibenclamide, is a Western medicine with obvious hypog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558
Inventor 周坚吴文晔熊晓芳
Owner 无锡安迪生物工程有限公司
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