Application of miRNA-29a compound as brain glioma marker

A technology of 1. mirna-29a, 2. mirna-29a, applied in the field of biomedical materials

Inactive Publication Date: 2011-04-27
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, whether miR-29a is an oncogenic factor or a tumor suppressor factor in cancer depends on the type of tumor and the cellular environment, and its role in glioma needs to be further studied.

Method used

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  • Application of miRNA-29a compound as brain glioma marker
  • Application of miRNA-29a compound as brain glioma marker
  • Application of miRNA-29a compound as brain glioma marker

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Experimental program
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Embodiment Construction

[0036] 1. Animal experiments

[0037] P53 knockout mice were purchased from the Model Animal Center of Nanjing University, and nude mice (BALB / cA-nu (nu / nu)) were purchased from the Shanghai Experimental Animal Center (Chinese Academy of Sciences, China), and were fed on a standard diet without pathogens.

[0038] 2. Glioma Clinical Sample Collection

[0039] Normal brain tissue samples and glioma tissue samples were obtained from Huashan Hospital Affiliated to Fudan University. After the surgery, the tissue block was cleaned with PBS or normal saline, and the tissue was quickly cut into small pieces, and some samples were put into 2ml cryopreservation tubes and stored in liquid nitrogen for later use. Others were fixed with paraformaldehyde and used for tissue section analysis. Samples for molecular analysis were ground into powders in liquid nitrogen to extract RNA or protein, respectively.

[0040] 3. RNA extraction:

[0041] Add 1 mL Trizol per 100 mg of tissue, and gr...

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Abstract

The invention belongs to the fields of biotechnology and medicine, in particular relates to an application of miR-29a as a novel brain glioma marker. The invention first validates that the expressions of the miR-29a in brain glioma tissues and cells are obviously decreased and the miR-29a acting as a tumor inhibiting gene is directly regulated and controlled by p53, and simultaneously first validates that p53 response genes such as MDM4, BCL2L2 and the like are direct targets of the miR-29a. Cytologic experiment proves that the miR-29a can obviously inhibit the propagation and infiltration of brain glioma cells, and animal in-vivo experiment proves that the miR-29a can inhibit the growth of the brain glioma. Besides, the further quantitative analysis of clinical brain glioma specimens of different levels shows that the miR-29a can be used as a brain glioma detection marker.

Description

technical field [0001] The invention relates to the use of a small molecule RNA, that is, miRNA-29a, and belongs to the technical field of biomedical materials. Background technique [0002] Glioma is the most frequent brain tumor in adults. From the time of diagnosis, the average life expectancy of glioma patients is less than one year. In order to treat this malignant disease, it is necessary to deeply explore its pathogenesis at the level of molecular biology. Recently, studies have reported that some small molecule microRNAs (miRNAs) are dysregulated in glioma and affect cell proliferation, apoptosis, invasion, metastasis and angiogenesis. These small noncoding RNAs include miR-21, miR-10b, miR-221 / 222, miR-26a, and miR-34a. Recent studies have found that miR-29a is highly expressed in normal tissues but downregulated in neuroblastoma. However, the research on miR-29a is different. There are reports that miR-29a is down-regulated in lung cancer and mantle cell lympho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A61K48/00A61P25/00A61P35/00
Inventor 赖立辉李丹刘顺英赵宇岚邹超张小龙
Owner EAST CHINA NORMAL UNIV
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