Application of miRNA-29a compound as brain glioma marker
A technology of 1. mirna-29a, 2. mirna-29a, applied in the field of biomedical materials
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[0036] 1. Animal experiments
[0037] P53 knockout mice were purchased from the Model Animal Center of Nanjing University, and nude mice (BALB / cA-nu (nu / nu)) were purchased from the Shanghai Experimental Animal Center (Chinese Academy of Sciences, China), and were fed on a standard diet without pathogens.
[0038] 2. Glioma Clinical Sample Collection
[0039] Normal brain tissue samples and glioma tissue samples were obtained from Huashan Hospital Affiliated to Fudan University. After the surgery, the tissue block was cleaned with PBS or normal saline, and the tissue was quickly cut into small pieces, and some samples were put into 2ml cryopreservation tubes and stored in liquid nitrogen for later use. Others were fixed with paraformaldehyde and used for tissue section analysis. Samples for molecular analysis were ground into powders in liquid nitrogen to extract RNA or protein, respectively.
[0040] 3. RNA extraction:
[0041] Add 1 mL Trizol per 100 mg of tissue, and gr...
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