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Analysis of transparent biological objects

A biological and object technology, applied in the field of analyzing at least one transparent biological sample, can solve problems such as limited information accuracy, achieve low-level noise, and reduce the rate of accidental interference

Inactive Publication Date: 2011-05-18
PHASE HOLOGRAPHIC IMAGING PHI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the quality of the hologram, and thus the accuracy of the information achieved by the method and apparatus, is limited

Method used

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  • Analysis of transparent biological objects
  • Analysis of transparent biological objects
  • Analysis of transparent biological objects

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Embodiment Construction

[0028] Such as figure 1 As shown, the observation vessel according to the invention comprises a first holding device 1 and a second holding device 2 . The first holding device 1 comprises a first outer surface 1a and a first inner surface 1b. The second holding means comprises a second inner surface 2a and a second outer surface 2b. The first inner surface 1b and the second inner surface 2a are arranged to hold and be in contact with a sample comprising at least one transparent biological object 3 and at least one medium 4 . also, figure 1 The object beam 5 and its relation to the first holding device 1 and the second holding device 2 are shown.

[0029] In one embodiment of the method according to the invention, mutually coherent at least one object beam and at least one reference beam of light are generated by splitting a beam originating from a coherent light source into two beams, eg by a beam splitter. The light beam originating from the coherent light source may be a...

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Abstract

The present invention relates to an observation vessel for digital holo-graphic microscopy of at least one transparent biological object, an observation vessel lid for digital holographic microscopy of at least one transparent biological object as well as a method for analyzing a sample comprising at least one transparent biological object and at least one medium by means of digital holographic microscopy.

Description

technical field [0001] The invention relates to an observation vessel for digital holographic microscopy of at least one transparent biological object, an observation vessel cover for digital holographic microscopy of at least one transparent biological object, and for analysis by digital holographic microscopy At least one method for transparent biological samples. Background technique [0002] There is an insatiable need for additional and more accurate information about biological objects such as cells in humans, animals, plants and other organisms. Cells have long been studied by light microscopy, such as fluorescence, confocal and phase contrast microscopy. However, when using fluorescence or confocal microscopy, cells must be labeled or stained, and the labels or stains used are potentially toxic to the cells, which may be affected and thus disrupt their development. In addition, markers can bleach over time, making the study of cell development difficult over a subs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G03H1/02G01L3/00G01B9/021
CPCB01L2300/0654G03H2001/005B01L2300/0829G02B21/00G03H1/0005G03H2001/0204B01L3/50853G03H2210/12G03H1/02G03H2210/63
Inventor 米卡埃尔·谢拜什陶安娜·默尔德伦纳特·伊塞尔松
Owner PHASE HOLOGRAPHIC IMAGING PHI
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