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Preparation of powder and injection preparations of echinacea purpurea polysaccharides

A technology of echinacea and powder injection, applied in the field of plant extracts and polysaccharide substances

Inactive Publication Date: 2011-06-22
QINGDAO VLAND BIOTECH INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Echinacea purpurea is used as an immunostimulator and immunomodulator in the Americas to treat acute or chronic respiratory tract infections caused by viral diseases or bacteria, and to assist in the treatment of various antibiotics and various serious bacterial infections. , cichoric acid and flavonoids and other active ingredients, but various preparations of Echinacea purpurea have not been used in the treatment of livestock and poultry diseases in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0043] Take the ultra-finely crushed Echinacea purpurea whole herb raw material, add 20 times the amount of water, heat and boil at 100°C for 3 hours, filter out impurities, and extract the clear liquid; then decolorize with activated carbon; after decolorization, the liquid is concentrated, and then precipitated with alcohol; Obtain the precipitate by centrifugation; then dry the precipitate, grind it, and pass through a 60-mesh sieve to obtain the crude polysaccharide; then add water to the crude polysaccharide, filter at a temperature of 4°C, and swell; after centrifugation, take the supernatant ; Then add Sevage reagent to remove protein, dialyze with deionized water; Washing twice; vacuum drying and grinding to obtain a white powdery substance, which is then dried to prepare a powder injection.

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Abstract

The method for preparing powder and injection preparations of echinacea polysaccharides comprises the following steps of: adding water into echinacea purpurea herb, boiling, extracting clear solution and decoloring; concentrating and performing ethanol precipitation; centrifuging precipitates; drying, grinding and screening to obtain crude polysaccharides; adding water into the crude polysaccharides, filtering and swelling; centrifuging, and taking supernatant; adding Sevage reagent into the supernatant to remove albumens, and dialyzing the solution by using deionized water; performing DEAE-Cellulose(DE-32) column chromatography; and eluting, concentrating, performing ethanol precipitation, centrifuging, ethanol washing and vacuum drying to obtain the powder and injection preparations of echinacea purpurea polysaccharides. The powder and injection preparations of echinacea purpurea polysaccharides can effectively enhance the immunity of livestock and poultry and improve the immunologic function of organisms.

Description

technical field [0001] The invention relates to a plant extract, in particular to a refined polysaccharide purified from Echinacea purpurea. The invention relates to an echinacea purpurea polysaccharide powder injection related to enhancing the immune function of livestock and poultry bodies, and belongs to the technical field of medicine. Background technique [0002] Echinacea (Echinacea) is a plant of the genus Echinacea native to North America and southern Canada. There are 8 species and several varieties in this genus. At present, there are mainly 3 varieties developed as medicine: Echinacea (Echinacea chinensis) purpurea, commonly known as echinacea), echinacea augustifolia (Echinacea augustifolia) and white echinacea (Echinacen pallida). At present, there are very few wild ones. They are native to North America and are now introduced and cultivated in Europe and other regions. In my country, Beijing, Nanjing, Shanghai and Changsha have also introduced and planted GA...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P37/02A61P37/04
Inventor 郝智慧曹蕾刘元元王春元张瑞丽邱梅
Owner QINGDAO VLAND BIOTECH INC
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