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Method for producing viral vaccines

A technology for the production of viruses and vaccines, applied in the direction of microorganism-based methods, biochemical equipment and methods, antiviral agents, etc., can solve the problems of low production efficiency, cell contamination, time-consuming and laborious cleaning and disinfection, etc., to improve production efficiency, cell Effect of high density, continuous harvest yield

Inactive Publication Date: 2013-01-23
BEIJING SKYWING TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, it can also solve the problems of time-consuming and laborious cleaning and disinfection of the reactor tank, low production efficiency, and easy cell contamination.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Using WAVE Bioreactor and FibraCel Disks Carrier to Cultivate CHO Cells to Produce Recombinant Hepatitis B Vaccine

[0062] Experimental equipment and experimental materials:

[0063] Bioreactor: WAVE Bioreactor System 200 (Life Science Department of General Electric Company), equipped with Cellbag-200L (Life Science Department of General Electric Medical Group);

[0064] Carrier: FibraCel Disks (New Brunswick Scientific Company) with a diameter of 6 mm;

[0065] Culture medium: DMEM medium (MD 208, Beijing Qingda Tianyi Biotechnology Co., Ltd.) supplemented with 5% calf serum.

[0066] The CHO cells used were CHO-C28 cell lines.

[0067] Steps:

[0068] Preparatory stage: Recover CHO-C28 cell line from the seed cell bank, scale up the culture in a spinner bottle, and digest it to make a cell suspension. Use an autoclave to sterilize the FibraCel Disks carrier and the culture medium at 121°C for 30 minutes by autoclaving.

[0069] Cell culture: add the st...

Embodiment 2

[0072] Example 2 Utilize WAVE bioreactor and FibraCel Disks carrier to cultivate Vero cells to produce rabies virus vaccine

[0073] Experimental equipment and experimental materials:

[0074] Bioreactor: WAVE bioreactor system 20 / 50 (Life Science Department of General Electric Company), equipped with Cellbag-50L (Life Science Department of General Electric Company);

[0075]Carrier: FibraCel Disks (New Brunswick Scientific Company) with a diameter of 6 mm;

[0076] Cell culture medium: MEM medium (MD505, Beijing Qingda Tianyi Biotechnology Co., Ltd.) supplemented with 5% calf serum.

[0077] The Vero cells used are ATCC line Vero cell strains. (purchased from the Chinese Type Culture Collection Center of Wuhan University)

[0078] Steps:

[0079] Preparatory stage: recover Vero cell lines from the seed cell bank, amplify the culture in a spinner bottle, and digest to make a cell suspension. Use a high-pressure steam sterilizer to sterilize the FibraCel Disks carrier and ...

Embodiment 3～5

[0083] Embodiment 3~5, comparative example 1, reference example 1~2

[0084] Except for changing the experimental conditions according to Table 1, the same operation as in Example 1 was carried out to obtain the hepatitis B vaccine, and the specific concentration results of the hepatitis B vaccine are shown in Table 1 together.

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PUM

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Abstract

The invention provides a method for producing viral vaccines, which comprises the following steps of: 1) inoculating cells for producing virus into a bioreactor with a Disks carrier, and culturing; and 2) harvesting the virus to prepare the vaccines. In addition, besides the step 1) of culturing the cells for producing the virus, the method further comprises the following steps of: when the cellsfor producing the virus grow to a certain density, inoculating and proliferating the virus to be produced. By the method, the cell yield can be improved, the production cost can be reduced, and the production efficiency can be improved.

Description

technical field [0001] The present invention relates to a method for the production of virus vaccines, in particular to the use of bioreactor and Disks vector method for large-scale production of viral vaccines. Background technique [0002] Usually, viral vaccines are produced by culturing animal cells on a large scale as host cells. At present, there are two types of culture systems for large-scale cultivation of animal cells, bioreactors and microcarriers. These two systems are usually combined for host cell culture. The combination of stirred bioreactors and spherical microcarriers is more commonly used. . Although the culture process combined with stirred bioreactor and spherical microcarrier has the advantages of monitoring the culture process and quality, stable production process, easy scale expansion, reduced pollution, easy control of product batch quality, and increased cell culture density. However, due to the limitations of the two systems, this culture pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12A61K39/205A61K39/13A61K39/29A61K39/135A61K39/20A61K39/187A61P31/12C12N7/00C12N5/07C12R1/93
CPCY02A50/30
Inventor 张韧陈文庆王建超刘俊生
Owner BEIJING SKYWING TECH CO LTD
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