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Antagonistic peptide of anti-opioid peptide and application thereof

A technology for anti-opioid peptides and antagonistic peptides, which is applied in the field of anti-opioid peptides and the application fields of the antagonistic peptides, and can solve the problems of acupuncture tolerance and the limited effective rate of acupuncture analgesia, etc.

Inactive Publication Date: 2012-07-04
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Professor Han Jisheng used an acupuncture treatment device to treat withdrawal symptoms, trying to stimulate the synthesis of endogenous opioids in the body through specific frequency electrical pulse stimulation to relieve symptoms, but its biggest defect is that the effectiveness of acupuncture analgesia in the population is limited and continues Acupuncture also has tolerance issues

Method used

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  • Antagonistic peptide of anti-opioid peptide and application thereof
  • Antagonistic peptide of anti-opioid peptide and application thereof
  • Antagonistic peptide of anti-opioid peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Example 1. Obtainment of anti-opioid peptide 99A and its functional verification

[0086] 1. Obtainment of porcine brain anti-opioid peptide 99A and detection of its dose-dependent effect

[0087] 1. Discovery of anti-opioid peptides

[0088] In the isolated mouse vas deferens specimens, stimulation with a square wave pulse (0.1MS width, 1 time / second) can cause contraction (see the literature for the specific method: J. Hughes et al (1975) Effect of morphine on adrenergic transmission in the Mouse Vas deferens.Assessment of agonist and antagonistpotencies of Narcotic analgesics 53,371-381), then, successively add morphine hydrochloride (3.2nM) or dog brain extract (AOS, 200μg / mL) (for dog brain extract The concentrated solution was successively filtered through Sephadex G-50 gel (purchased from Pharmacia Company), and after cation exchange chromatography on carboxymethyl-cellulose (CM-C was purchased from H.Reeve-Angel&.Co.Ltd Company), the sample was obtained) , to ...

Embodiment 2

[0117]Example 2. Obtaining and Activity Detection of Anti-opioid Peptide 99A Antagonist Peptide

[0118] 1. Obtaining antagonistic peptides of 99A-16, 99A-19 and DBI-16

[0119] According to the principle of interaction between protein molecules, the reference (J.R.Heal et al Specific interactions between sense and complementary peptides: The Basis for the proteomic code ChemBIOChem 2002, 3, 114-131) designed 99A-16, 99A-19 and DBI -16 antagonistic peptides of three short peptides, wherein the antagonistic peptide of 99A-16 is named CP-99A-16+5, has the amino acid residue sequence of SEQ ID NO: 5, consists of 21 amino acid residues, and The coding sequence has the nucleotide sequence of SEQ ID NO: 8 in the sequence table, consisting of 63 bases; the antagonistic peptide of 99A-19 is named CP-99A-19, and has the amino acid residue sequence of SEQ ID NO: 6 , consisting of 19 amino acid residues, its coding sequence has the nucleotide sequence of SEQ ID NO: 9 in the sequence tab...

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Abstract

The invention discloses an anti-opioid peptide, as well as an antagonistic peptide and application thereof. The anti-opioid peptide is one of the following amino acid residue sequences: (1) SEQ ID NO:1 in a sequence table and (2) a polypeptide which is obtained by substituting, deleting or adding 1-10 amino acid residues on an amino acid residue sequence of SEQ ID NO:1 in the sequence table and has an antinociceptic effect on antagonistic morphine. The antagonistic peptide is one of the following amino acid residue sequences: (1) SEQ ID NO:5-7 in a sequence table and (2) a polypeptide which is obtained by substituting, deleting or adding 1-10 amino acid residues on an amino acid residue sequence of SEQ ID NO:5-7 in the sequence table and has the effects of enhancing morphine analgesia, reversing morphine tolerance and / or weakening or eliminating abstinence syndromes. The anti-opioid peptide as well as an active segment and the antagonistic peptide thereof play an important role in thefield of preparation of medical and medicament abstinence medicaments, and have wide application prospects.

Description

[0001] This application is a divisional application with the application number 200710100384.4, the filing date is June 11, 2007, and the title of the invention is "anti-opioid peptide and its antagonistic peptide and the application of the antagonistic peptide". technical field [0002] The present invention relates to peptides and their coding genes and applications, in particular to an anti-opioid peptide and its antagonistic peptide which can antagonize the antinociceptive effect of morphine, and the antagonistic peptide can enhance morphine analgesia, reverse morphine tolerance and / or Use in drugs that reduce or eliminate the effects of withdrawal symptoms. Background technique [0003] As we all know, rampant drug trafficking and drug use around the world has become a cancer that endangers society, seriously interfering with orderly economic construction and social stability. In addition, intravenous drug use has also caused a rise in the infection rate of AIDS populati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C12N15/11C12N15/63C12N5/10A61K38/10A61P25/36A61P25/04
Inventor 陈玉珍吴才宏曲红
Owner PEKING UNIV
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