Compound biological control agent as well as preparation method and application thereof

Abstract

The invention discloses a compound biological control agent and an application thereof, relating to the compound technology of extracting plant tissue fluids and self-sieving strains. The self-sieving strain MDCGTH18 used by the compound biological control agent has the registering number of CGMCC (China General Microbiological Culture Collection) NO.2965. After ailanthus leaves and oleander are compounded with the bacterial strain, various germs can be effectively prevented from breeding; meanwhile, defense reaction can be induced in a plant body, so that the plant body has disease-resistantcapability; the compound biological control agent has strong bacteriostatic activity on pathogenic fungi, bacteria and nematode of various plants, can be used for the biological control of various plant diseases and has the excellent effects of strong bacteriostatic activity and wide antibacterial spectrum.

Description

Technical field:

[0001] The invention belongs to the field of biological technology, and relates to a biocontrol agent compounded with plant extracts and biological bacteria, a preparation method thereof and an application in preventing and treating plant diseases. technical background:

[0002] Worldwide, plant diseases commonly cause severe losses to agricultural production. Now take rice root nematode (Hirschmannielaspp.) as an example, root nematode is an important parasitic nematode disease of rice, mainly infects rice roots, directly affects rice tillering, it is estimated that 58% of rice fields in the world are seriously infringed by potential root nematodes , The yield loss reaches 25%, and the severe one reaches 40%. At present, chemical control is still the most important control measure for plant parasitic nematode diseases, but traditional chemical nematicides such as chloropicrin, methyl bromide, carbofuran, gramphos, methomyl, etc. have high cost of use and p...

Examples

Embodiment 1

[0060] Comparison of Nematicidal Activity of Tissue Extracts from Different Parts of Ailanthus Ailanthus and Oleander

[0061] Extract the tissue fluid of Ailanthus bark, leaf and oleander flower, leaf and skin respectively, insert nematodes into different extracts respectively, count the survival of nematodes after 24 hours, count the number of survivals and deaths of nematodes, and calculate Mortality rate, the average mortality rate of nematodes in the plant extract for 24 hours is 0-10.0%, the measurement result is represented by "one"; the mortality rate is 10.1%-30.0%, the measurement result is represented by "+"; the mortality rate is 30.1% ~50.0%, the measurement result is represented by “++”; the mortality rate is 50.1%~80.0%, the measurement result is represented by “+++”; the mortality rate is 80.1%~100%, the measurement result is represented by “++” ++" means.

[0062] The results are shown in Table 1:

[0063] Table 1 The nematicidal effect of extracts from diff...

Embodiment 2

[0068] Evaluation of insecticidal effect after compounding with different proportions

[0069] The leaves of Ailanthus ailanthii and the flower extracts of oleander were used for compounding in different proportions, and the nematicidal effect after compounding was observed. The results are shown in Table 2.

[0070] Table 2 Comparison of insecticidal effects of different compound ratios

[0071]

[0072] Experimental results show that when the ratio of Ailanthus sinensis and oleander extract is 1:2, the nematode killing effect is the best, so this ratio is preferred for the best compounding effect.

Embodiment 3

[0074] Composite test of two kinds of extracts and self-screening strains

[0075] The best compounding plan obtained from the above experiments, that is, Ailanthus ailanthus and oleander extract was compounded at a ratio of 1:2, and then compounded with the self-screening strain MDCGTH 18 to observe the effect of nematodes and disease prevention. The experimental results are shown in Table 3.

[0076] Table 3 Comparison of insecticidal effects of three kinds of material compound preparations

[0077]

[0078] From the test results shown in the table above, it can be seen that the insecticidal effect of compounding the Ailanthus extract, the oleander extract and the self-screening strain MDCGTH 18 fermentation culture is better than that of simply using the Ailanthus extract and the oleander flower extract. The insecticidal effect was good after the compounding of the three substances; and the insecticidal effect of the compounding of the three substances in different prop...

Technical field:

[0001] The invention belongs to the field of biological technology, and relates to a biocontrol agent compounded with plant extracts and biological bacteria, a preparation method thereof and an application in preventing and treating plant diseases. technical background:

[0002] Worldwide, plant diseases commonly cause severe losses to agricultural production. Now take rice root nematode (Hirschmannielaspp.) as an example, root nematode is an important parasitic nematode disease of rice, mainly infects rice roots, directly affects rice tillering, it is estimated that 58% of rice fields in the world are seriously infringed by potential root nematodes , The yield loss reaches 25%, and the severe one reaches 40%. At present, chemical control is still the most important control measure for plant parasitic nematode diseases, but traditional chemical nematicides such as chloropicrin, methyl bromide, carbofuran, gramphos, methomyl, etc. have high cost of use and p...

Examples

Embodiment 1

[0060] Comparison of Nematicidal Activity of Tissue Extracts from Different Parts of Ailanthus Ailanthus and Oleander

[0061] Extract the tissue fluid of Ailanthus bark, leaf and oleander flower, leaf and skin respectively, insert nematodes into different extracts respectively, count the survival of nematodes after 24 hours, count the number of survivals and deaths of nematodes, and calculate Mortality rate, the average mortality rate of nematodes in the plant extract for 24 hours is 0-10.0%, the measurement result is represented by "one"; the mortality rate is 10.1%-30.0%, the measurement result is represented by "+"; the mortality rate is 30.1% ~50.0%, the measurement result is represented by “++”; the mortality rate is 50.1%~80.0%, the measurement result is represented by “+++”; the mortality rate is 80.1%~100%, the measurement result is represented by “++” ++" means.

[0062] The results are shown in Table 1:

[0063] Table 1 The nematicidal effect of extracts from diff...

Embodiment 2

[0068] Evaluation of insecticidal effect after compounding with different proportions

[0069] The leaves of Ailanthus ailanthii and the flower extracts of oleander were used for compounding in different proportions, and the nematicidal effect after compounding was observed. The results are shown in Table 2.

[0070] Table 2 Comparison of insecticidal effects of different compound ratios

[0071]

[0072] Experimental results show that when the ratio of Ailanthus sinensis and oleander extract is 1:2, the nematode killing effect is the best, so this ratio is preferred for the best compounding effect.

Embodiment 3

[0074] Composite test of two kinds of extracts and self-screening strains

[0075] The best compounding plan obtained from the above experiments, that is, Ailanthus ailanthus and oleander extract was compounded at a ratio of 1:2, and then compounded with the self-screening strain MDCGTH 18 to observe the effect of nematodes and disease prevention. The experimental results are shown in Table 3.

[0076] Table 3 Comparison of insecticidal effects of three kinds of material compound preparations

[0077]

[0078] From the test results shown in the table above, it can be seen that the insecticidal effect of compounding the Ailanthus extract, the oleander extract and the self-screening strain MDCGTH 18 fermentation culture is better than that of simply using the Ailanthus extract and the oleander flower extract. The insecticidal effect was good after the compounding of the three substances; and the insecticidal effect of the compounding of the three substances in different prop...

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