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Cerasus campanulata somatic cell embryogenesis method

A technology of embryogenesis and somatic cells, applied in the field of plant cultivation, to achieve the effects of complete structure, fast seedling growth and short cycle

Active Publication Date: 2011-10-19
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the organogenesis of Fujian mountain cherry has been relatively systematic, but these studies are not yet able to achieve rapid cultivation of Fujian mountain cherry to meet the use requirements

Method used

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  • Cerasus campanulata somatic cell embryogenesis method
  • Cerasus campanulata somatic cell embryogenesis method
  • Cerasus campanulata somatic cell embryogenesis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Induction of embryogenic callus

[0034]Immature embryos were used to induce embryogenic callus. The immature fruits collected in spring are first washed with running water for 2 hours, then sterilized with alcohol for 30 seconds on an ultra-clean bench, rinsed with sterilized distilled water, and then treated with 0.1% mercury chloride or 84 disinfectant for 7-20 minutes, and rinsed with distilled water , the immature fruit is cut open with a scalpel in the ultra-clean workbench, and two cotyledons and immature embryos are taken out and placed on the induction medium, such as figure 2 As indicated, the inoculated material was placed in an incubator at 23°C for dark cultivation. The immature embryo starts to start about half a month after being placed on the induction medium, such as image 3 , Figure 4 , Figure 5 and Figure 6 As shown in , some have direct somatic embryogenesis, some have red radicles that begin to elongate, some have white protrusio...

Embodiment 2

[0036] Embodiment 2 Embryogenic callus proliferation stage

[0037] In the embryogenic callus proliferation stage, the proliferation medium was used, and the proliferation medium continued to use MS basic medium, and placed in an incubator at 23°C for dark culture, such as Figure 7 shown. Embryoid body generation, on the embryogenic callus proliferation medium, after several subcultures, as Figure 8 and Figure 9 As shown, white, spherical embryoid bodies were produced on yellow-green callus.

Embodiment 3

[0038] The development and maturation of embodiment 3 embryoid bodies

[0039] After the development and maturation of the embryoid body, after the formation of the spherical embryo, transfer it to the cultivation medium for cultivation under light, and light for 12 hours d －1 , the illuminance is 1200 lx, and the room temperature is 22-27°C. Such as Figure 10 As shown, the seedlings developed into plantlets on the MS medium, transferred to the growth medium, continued to cultivate, and grew into complete plantlets, such as Figure 11 shown.

[0040] The somatic embryogenesis technology of Fujian mountain cherry of the present invention provides a method for the large-scale vegetative propagation of Fujian mountain cherry with a short cycle, high reproduction rate and low cost, and effectively solves the problem that the existing stands of Fujian mountain cherry are extremely rare and the seeds are mature. It is very easy to burst, the collection, storage and germination...

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Abstract

The invention discloses a cerasus campanulata somatic cell embryogenesis method, comprising the following steps: inducting embryogenic callus of immature embryos in an induction medium; using a multiplication medium to multiply the embryogenic callus; and using a cultivation medium and a growth medium to cultivate embryoids, such that the embryoids grow and mature; finally, complete sprouts are obtained. The cerasus campanulata somatic cell embryogenesis method provided by the present invention assists in providing a cerasus campanulata vegetative propagation method with short period, high reproduction rate, and low cost. Presently, existing cerasus campanulata groves are rare; seed explosion is likely to occur during seed maturation; seed harvesting, storing and germinating are difficult; traditional seed breeding method is difficult to apply; traditional seed breeding efficiency is low; and landscaping and production requirements can not be satisfied. With the method provided by the present invention, the problems can be solved.

Description

technical field [0001] The invention belongs to plant cultivation technology, and relates to a method for somatic embryogenesis of Fujian mountain cherry blossoms. Background technique [0002] Fujian mountain cherry blossoms ( Cerasus campanulata Maxim.) belongs to Rosaceae (Rosaceae) Prunusoidea (Cerasus), also known as Feihan cherry, Fujian cherry, mountain cherry, bell flower cherry, knot cherry, etc., deciduous tree, up to 15m; bark taupe Or dark brown, rough without cracking, tough texture, and lateral peeling. Leaves are papery, ovate, elliptic-ovate or ovate-oblong, 4-9cm long, 2.5-3.5cm wide, apex acuminate, base nearly round or slightly cordate, with sharp and thin single or micro-aliasing. There are a pair of red glands, the single leaves are spiral and alternate, double serrate margin (double serrate margin), crushed with almond flavor; stipules are linear and lobed. Blooms in late winter and early spring every year, first flowers and then leaves, flowers are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 施季森张元莉陈金慧苏倩吕月良陈璋
Owner NANJING FORESTRY UNIV
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