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Three-dimension microscopic observation method of plant leaf stoma in vivo

A technology of plant leaves and stomata, which is applied in the field of three-dimensional microscopic observation of plant leaf stomata in vivo, and can solve problems such as construction

Inactive Publication Date: 2012-10-03
CHINA JILIANG UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, how to construct the three-dimensional shape of the observed object from two-dimensional images and information is a technical problem

Method used

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  • Three-dimension microscopic observation method of plant leaf stoma in vivo
  • Three-dimension microscopic observation method of plant leaf stoma in vivo
  • Three-dimension microscopic observation method of plant leaf stoma in vivo

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Embodiment Construction

[0028] Such as figure 1 As shown, after the in vivo microscopic observation experiment of plant leaf stomata was selected using this measurement method, the optical magnification components were installed first, including the long working distance metallographic objective lens and the zoom objective lens. Secondly, clamp the blade to be tested, and use the magnets on the front and rear splints to attract and close to ensure that the blade surface in the observation area is flat. Set relevant parameters on the host computer, such as sampling frequency, acquisition time, etc. Then carry out automatic or manual focusing, so that the long working distance metallographic objective lens is facing the stomatal observation area of ​​the plant leaf, and adjust until the field of view is clear and bright. In this way, the two-dimensional image of the stomata can be collected. After performing steps such as image denoising, curve smoothing, and curve fitting on the collected two-dimens...

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Abstract

The invention relates to a three-dimension microscopic observation method of plant leaf stoma in vivo. The method comprises the following steps that: firstly, optical amplifier components are connected with a focusing guide rail and a varifocus lens is connected with a CCD image acquisition sensor; the focusing guide rail is connected with a connection plate and the connection plate is connected with a horizontal fine tuning guide rail; then, a measured plant is placed on a measured-object load-bearing platform and a observation area of a measured leaf is fixed; the CCD image acquisition sensor is connected with an upper computer so as to real-timely collecting and observing leaf stomata through image acquisition software; finally, an image of a leaf surface can be acquired through automatically focusing or manually focusing and a three dimensional image of leaf surface stoma states can be obtained after performing image denosing, curve smoothing and curve fitting on collected data. By using the method of the invention, when the plant leaves are not separated with the plant body, stoma state images can be continuously collected, and normal plant growth state can be two-dimensionally observed or leaf stoma change status under a specific experiment state can be two-dimensionally observed. Therefore, maximum information of the leaf stomata can be guaranteed.

Description

technical field [0001] The invention belongs to the fields of biological detection technology and optical image construction, and relates to a three-dimensional microscopic observation method of stomatal living bodies of plant leaves. Background technique [0002] As an important organ of the plant, the leaf can reflect the condition of the plant more sensitively than other organs, so the physiological response of the leaf has attracted much attention. Stomata are one of the important components of plant leaves. They are composed of two guard cells. The size of the cells is on the order of microns. They are the CO in the air. 2 It is the main channel for the evaporation of water into the plant body and the plant body. The stomata of plant leaves mainly control two physiological processes of plants, namely controlling the absorption of CO in photosynthesis 2 Molecular balance and control of loss of water molecules during transpiration. In addition, during the diffusion of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01B11/24
Inventor 李东升郭天太郭琳陈爱军张雯郭冲冲
Owner CHINA JILIANG UNIV
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