Quantitation concentrator of noctiluca scientillans living cells

Abstract

The invention discloses a quantitation concentrator of noctiluca scientillans living cells. The quantitation concentrator comprises a quantitating cylinder with a bottom and a collecting cylinder without a bottom. A positioning sleeve is arranged in the quantitating cylinder. The positioning sleeve is provided with fixing feet fixedly connected to the bottom of the quantitating cylinder. The collecting cylinder is movably connected to the positioning sleeve. Sieving gauze with apertures of 80 to 180 micrometers is arranged between the collecting cylinder and the positioning sleeve. The cylinder port of the quantitating cylinder is higher than the upper rim of the positioning sleeve, and is lower than the port of the collecting cylinder. Therefore, a quantitated noctiluca scientillans living cell water sample is slowly poured into the collecting cylinder; most of the water content in the noctiluca scientillans living cell water sample flows into the quantitating cylinder and is over-flown from the cylinder port; noctiluca scientillans living cells are separated by the sieving gauze, and are kept in the collecting cylinder, such that the noctiluca scientillans living cells are concentrated. The density of the noctiluca scientillans living cells is high, and a uniformly distributed suspension is formed. Collapse and declination of the living cells are rarely occurred. Therefore, the quantitative concentrator provided by the invention is beneficial for the quantitative calculation of noctiluca scientillans living cells, and for the researches of crucial information substances in noctiluca scientillans living cell division, noctiluca scientillans cell life history, and noctiluca scientillans population proliferation.

Description

technical field [0001] The invention relates to an algae concentrating device, in particular to a quantitative concentrator for living cells of nocturnal algae. Background technique [0002] Noctiluca scintillans or N. miliaris, also known as Noctiluca scintillans or N. miliaris, is a heterotrophic unicellular planktonic dinoflagellate species. The vegetative cells are usually spherical, with a diameter of 200 μm to 2000 μm. Noctiluca is a harmful planktonic dinoflagellate species that often causes red tides. Red tides have been reported in sea areas and aquaculture environments around the world. The research progress on key issues of noctiluca red tides has been relatively slow. One important reason is the relevant Research on algal cell division, cell life history and its significance, key information substances and mechanism of action in the process of population proliferation is quite lacking. At present, the usual monitoring method is to record and screen the density o...

AI Technical Summary

Problems solved by technology

At present, the usual monitoring method is to record and screen the density of nocturnal algae in fixed water samples. This is a heavy workload, and it is difficult to obtain a sufficient amount of nocturnal algae cells, which directly affects the field and indoor research on nocturnal algae. The study of living cells is subject to extreme research constraints

Under natural conditions, the acquisition of a large number of noctiluca cells can only be achieved in a high-density aggregation state, which is difficult to collect. Even if it can be collected, the opportunity to study the in situ characteristics of the cells before aggregation has been missed.

In indoor research, it is a heavy workload and low efficiency to record the nocturnal algae in all the water samples in the petri dish one by one, and because of the low density of the water samples and the floating characteristics of the nocturnal algae, it is difficult to form a uniform distribution state of the water samples. Inconvenience caused by key information substances in the process of algal cell division, cell life history and population proliferation

Although the invention patent application with the publication number CN102102083 discloses a cyanobacteria concentrating device, an air buoy, a collection cylinder and a stirring device are arranged in the tank, which allows the cyanobacteria to float up through the air flotation principle, and the floating cyanobacteria is stirred evenly by the stirring device, and enters the collection cylinder It is discharged from the drain pipe, but the purpose of this device is to destructively remove some cyanobacteria, reduce the load on the compressor, and cannot be used in the research of algae metering and concentration, let alone the concentration and quantification of living microalgae-unicellular algae

There is no technology and device for concentrating and quantifying Nocturnal algae disclosed at present

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