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Carbazole-degrading bacterium and its culture method

A technology of carbazole and pseudomonas, applied in the direction of bacteria, chemical instruments and methods, and methods based on microorganisms, can solve the problems of inactivation and failure of common microbial populations, and achieve simple cultivation methods, strong degradation activity, and growth fast effect

Inactive Publication Date: 2013-03-20
PEKING UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional wastewater biological treatment uses a large number of natural or domesticated microbial populations to effectively remove easily degradable organic pollutants under the working conditions of the treatment system; however, with the rapid development of modern industry, more and more More and more wastewater contains a variety of toxic and harmful substances, resulting in the inactivation and failure of most common microbial populations in traditional biological treatment systems

Method used

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  • Carbazole-degrading bacterium and its culture method
  • Carbazole-degrading bacterium and its culture method
  • Carbazole-degrading bacterium and its culture method

Examples

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Embodiment 1

[0038] Example 1. Isolation, purification, identification and drug resistance of Pseudomonas sp. BC039

[0039] 1. Separation and purification

[0040] Pseudomonas sp. BC039 is a strain of G-bacteria isolated and purified from coking activated sludge. Carbazole is the only available carbon and nitrogen source. Strain BC039 was obtained from long-term domesticated activated sludge through enrichment, separation, screening, and purification steps.

[0041] The specific separation process is as follows: inoculate the collected coking activated sludge sample into the liquid MSM selective medium containing carbazole (the medium is filled with carbazole as the only carbon source and nitrogen source available for microorganisms, and DMSO dissolved carbazole carbazole+MSM) in shake flasks, 30°C, 180rpm, shaking culture for five cycles, each cycle about 3d, the concentration of carbazole in the five cycles was 100, 200, 300, 500, 500 mg / L. Dilute the enrichment solution to 10 -7 tim...

Embodiment 2

[0059] Example 2, Functional Research of Pseudomonas sp. BC039

[0060] 1. Biodegradation of carbazole by Pseudomonas sp. BC039

[0061] 1. Biodegradation of carbazole by Pseudomonas sp. BC039

[0062] Pick the monoclonal Pseudomonas sp. BC039 from the MSM solid medium, inoculate it in 100 mL of LB liquid medium, shake it at 30°C and 180 rpm (amplitude 26 mm) for 36 hours, and centrifuge at 4000 rpm for 10 minutes to collect Bacteria; then wash the bacterium with sterile liquid MSM to remove residual LB medium and bacterial metabolic impurities; then collect the bacterium by centrifuging at 4000rpm for 10min; finally resuspend the obtained bacterium with MSM aqueous solution to prepare bacterial suspension.

[0063] Prepare 100 mL of MSM selective medium containing carbazole concentration of 520.6 mg / L, the initial pH value is 7.0, and inoculate the above-prepared Pseudomonas sp. BC039 bacterial suspension (the amount of bacteria is about 0.04 g / L) At the same time, a blank...

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Abstract

The invention discloses a strain of carbazole-degrading bacterium and its culture method. The collection number of the Pseudomonas sp. BC039 provided by the invention is CGMCC No.4222. The invention also provides an application of the Pseudomonas sp. BC039 CGMCC No.4222 in the degradation of carbazole. It is proved through an experiment that the Pseudomonas sp. BC039 provided by the invention can be used as a bioaugmentation agent in the hardly-degraded organic wastewater biological treatment technology. In addition, corresponding environmental protection biological preparations developed from the Pseudomonas sp. BC039 has great research, application and market values.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a carbazole-degrading bacterium and a culture method thereof. Background technique [0002] Inspired by the biological action of the earth's self-purification process, which has the advantages of low cost, light secondary pollution, and good environmental compatibility, the biological treatment technology of treating wastewater with microorganisms has attracted increasing attention. In biological treatment technology, microbial populations with highly active degradation functions play an important role. Traditional wastewater biological treatment uses a large number of natural or domesticated microbial populations to effectively remove easily degradable organic pollutants under the working conditions of the treatment system; however, with the rapid development of modern industry, more and more More and more wastewater contains a variety of toxic and harmful substances, resulting in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34C12R1/38A62D101/26C02F101/38
Inventor 温东辉赵翠唐孝炎
Owner PEKING UNIV
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