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Paracoccus and application thereof in nitrogenous heterocyclic compound degradation

A nitrogen heterocyclic compound, paracoccus technology, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of pollution and three wastes, which are difficult to achieve effective treatment effects, and achieve fast growth rate, strong degradation activity, The effect of the simple method of cultivation

Inactive Publication Date: 2010-04-07
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing commercialized biological preparations are difficult to effectively control the "three wastes", and serious pollution needs to be solved urgently

Method used

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  • Paracoccus and application thereof in nitrogenous heterocyclic compound degradation
  • Paracoccus and application thereof in nitrogenous heterocyclic compound degradation
  • Paracoccus and application thereof in nitrogenous heterocyclic compound degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Isolation, purification and identification of Paracoccus sp. BW001 CGMCC No. 2225

[0026] 1. Isolation, purification and identification of Paracoccus sp. BW001 CGMCC №.2225

[0027] Paracoccus sp. (Paracoccus sp.) BW001 CGMCC №.2225 is a leather strain obtained from the activated sludge collected from the aeration tank of the wastewater treatment plant of the coking plant of Wuhan Iron and Steel Group, and the activated sludge was domesticated and cultivated. For Lambert-negative bacteria, the specific process of enrichment, separation and purification is as follows:

[0028] Under the condition of selective pressure pyridine 500mg / L, that is, using pyridine as the only carbon and nitrogen source, Paracoccus sp. was obtained from the activated sludge domesticated for a long time through enrichment, separation, screening, purification and other steps. BW001. The specific separation process is as follows:

[0029] The collected activated sludge samples were...

Embodiment 2

[0043] Example 2. Detection of Pyridine Biodegradation by Paracoccus sp. BW001 CGMCC №.2225 and Screening of Degradation Conditions

[0044] 1. Detection of pyridine biodegradation by Paracoccus sp. BW001 CGMCC №.2225

[0045] 1) Preparation of Paracoccus sp. BW001 CGMCC №.2225 bacterial suspension

[0046] Pick 2 rings of Paracoccus sp. BW001 cultured on the slant and inoculate them into a 250mL Erlenmeyer flask filled with 50mL LB liquid medium, culture at 30°C with shaking at 180rpm for 36h, and centrifuge at 4000rpm for 10min to collect the bacteria; then use Wash the bacterium liquid with sterile normal saline, then centrifuge, and repeat this twice, and the centrifugation condition is also 4000rpm, 10min; Finally, the gained thalline is resuspended with MSM aqueous solution to prepare a bacterium suspension (concentration is 10 8 ~10 9 cfu / mL).

[0047] 2) Detection of pyridine biodegradation by Paracoccus sp. BW001 CGMCC №.2225 Inoculate the paracoccus sp. BW001 bact...

Embodiment 3

[0063] Example 3, Plasmid Detection of Paracoccus sp. BW001

[0064] Using the improved alkaline denaturation method, the plasmid was extracted from the culture solution of BW001 bacteria. The specific plasmid extraction process was as follows:

[0065] 1. Materials used:

[0066] (1) Culture medium: LB medium

[0067] (2) Solution used for plasmid extraction:

[0068] P1: Dissolve 6.06g Tris base, 3.72g Na 2 EDTA·2H 2 O in 800 mL of distilled water, adjust the pH to 8.0 with HCl, make up to 1 L with distilled water, and add 100 mg of RNase A.

[0069] P2: Dissolve 8.0g NaOH in 950mL distilled water, 50mL 20% SDS (w / v) solution, distilled water to 1L.

[0070] P3: Dissolve 294.5 g of potassium acetate in 500 mL of distilled water. Adjust the pH to 5.5 with glacial acetic acid (about 110 mL), and distill the volume to 1 L with distilled water.

[0071] TE buffer: add 1.576g Tris·Cl per liter of distilled water to adjust the pH to 8.0; add 0.3722g EDTA·Na 2 .

[0072] 2...

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Abstract

The invention discloses paracoccus and the application for degrading azotic heterocyclic compound. The paracoccus is paracoccus sp. BW001 CGMCC No. 2225. The paracoccus sp. BW001 CGMCC No. 2225 of theinvention, on one hand, can grow into pyridine as the only carbon source and nitrogen source, which has a strong degradation activity (the degradation rate is 100%) for pyridine, is extremely suitablefor biologic control of organic wastewater (such as coking wastewater) which contains pollutant of pyridine category, and can be used as biological hardening agent in the biological control technolog y of organic persistent wastewater, and corresponding environmental biological agent is exploited, thereby having relatively high value of study, application and marketing.

Description

technical field [0001] The invention relates to a strain of paracoccus and its application in degrading nitrogen-containing heterocyclic compounds. Background technique [0002] Inspired by the "self-purification" process of the earth, biological treatment has the advantages of low cost, light secondary pollution, and good environmental compatibility. The research and application of microbial wastewater treatment technology has attracted more and more attention. Functional microbial groups play an important role in this technology. The traditional biological method of treating wastewater uses natural or domesticated microbial flora, which are allowed to degrade naturally. The degradation rate of pollutants is not high, which can no longer adapt to today's More and more types of industrial wastewater. Therefore, the isolation and screening of special microorganisms, the isolation and extraction of functional genes, and the construction of various environmental engineering ba...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
Inventor 温东辉赵翠孙庆华柏耀辉唐孝炎
Owner PEKING UNIV
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