Pseudomonas and application thereof in carbazole degradation

A technology of Pseudomonas and carbazole, applied to Pseudomonas and its application in carbazole degradation, can solve problems such as slow chemoautotrophic nitrification, achieve strong degradation activity, fast growth, The effect of cultivation method is simple

Inactive Publication Date: 2012-12-19
PEKING UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biological denitrification is limited by various conditions and factors. For example, the chemoautotrophic nitrification rate of nitrous bacteria is slow, and the aerobic and anoxic denitrification processes make it necessary to set different dissolved oxygen environments during the denitrification process.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pseudomonas and application thereof in carbazole degradation
  • Pseudomonas and application thereof in carbazole degradation
  • Pseudomonas and application thereof in carbazole degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. Isolation, purification and identification of Pseudomonas sp. BC046

[0038] 1. Separation and purification

[0039] Pseudomonas sp. BC046 is a strain of G isolated and purified from coking activated sludge - Bacteria, under the condition of a certain concentration of carbazole (DMSO dissolved state) as the selection pressure, that is, carbazole as the only available carbon source and nitrogen source, are enriched and separated from the activated sludge domesticated for a long time. , screening, purification and other steps to obtain BC046.

[0040] The specific separation process is as follows: inoculate the collected coking activated sludge sample into the liquid MSM selective medium containing carbazole (the medium is filled with carbazole as the only carbon source and nitrogen source available for microorganisms, and DMSO dissolved carbazole carbazole+MSM) in shake flasks, 30°C, 180rpm, shaking culture for five cycles, each cycle about 3d, the concentra...

Embodiment 2

[0051] Example 2, Functional Research of Pseudomonas sp. BC046

[0052] 1. Degradation of carbazole by Pseudomonas sp. BC046

[0053] 1. Preparation of Pseudomonas sp. BC046 bacterial suspension

[0054] Pick a single colony of Pseudomonas sp. BC046 from the MSM solid medium and inoculate it in 100 mL of LB liquid medium, shake it at 30°C and 180 rpm (amplitude 26 mm) for 36 hours, and centrifuge at 4000 rpm for 10 minutes to collect the bacteria; Then wash the bacterial solution with sterile normal saline to remove the residual LB medium and impurities metabolized by the bacterial cells; then collect the bacterial cells by centrifugation at 4000rpm for 10 minutes; finally resuspend the obtained bacterial cells with MSM aqueous solution to prepare bacterial cells Suspension.

[0055] 2. Optimal environmental conditions for the degradation of carbazole by Pseudomonas sp. BC046

[0056] Investigate three environmental factors that affect the degradation of Pseudomonas sp. BC0...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a pseudomonas for carbazole degradation and a culturing method thereof. The pseudomonas (Pseudomonas sp.) BC 046 provided by the invention has a preservation number CGMCC No.4223; the invention also provides an application of pseudomonas (Pseudomonas sp.) BC 046 CGMCC No.4223 in preparing product of carbazole degradation or provides an application of pseudomonas (Pseudomonas sp.) BC 046 CGMCC No.4223 in carbazole degradation. The experiment of the invention proves that the pseudomonas (Pseudomonas sp.) BC 046 provided by the invention can be used as biological enhancerin biological treatment process of refractory organic wastewater; furthermore, corresponding environmental biological agent can be developed; the invention has high research, application and market value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of pseudomonas and its application in carbazole degradation. Background technique [0002] my country has a lot of work foundations in water pollution control, but the treatment of organic industrial wastewater is still very serious. Among many industrial wastewater treatment technologies, the biological treatment method has attracted people's attention because of its advantages such as low cost, light secondary pollution, and good environmental compatibility. Functional microbial populations play an important role in this technology. The traditional biological treatment of wastewater utilizes natural or domesticated microbial flora and allows them to degrade naturally. The degradation rate of pollutants is not high, and it is no longer suitable for today's increasingly More and more types of industrial wastewater. Biotechnology is the most mature and challenging technolog...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/38C02F101/16C02F101/38A62D101/26
Inventor 温东辉赵翠郑中原唐孝炎
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap