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Microbe-enzyme composite preparation used for restoring water in urban and rural polluted river, and its preparation method

A compound preparation and water body restoration technology, applied in the field of environmental protection, can solve the problems of ineffective removal of ammonia nitrogen inorganic pollutants, weak microbial metabolic activity, low environmental tolerance, etc., and achieve low cost, quick results, and low operating costs.

Active Publication Date: 2013-12-25
浙江绿凯环保科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For potassium permanganate index (COD Mn ) and ammonia nitrogen as the main pollutants in urban and rural river water bodies, although the restoration by adding compound enzyme bioactivators or adding functional microbial flora has achieved certain results, the problem is that the compound enzyme bioactivators The effect of the method cannot last long, and it cannot effectively remove inorganic pollutants such as ammonia nitrogen, and the method of adding functional microbial flora faces problems such as weak microbial metabolic activity and low environmental tolerance during the application process.

Method used

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  • Microbe-enzyme composite preparation used for restoring water in urban and rural polluted river, and its preparation method
  • Microbe-enzyme composite preparation used for restoring water in urban and rural polluted river, and its preparation method
  • Microbe-enzyme composite preparation used for restoring water in urban and rural polluted river, and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Slant activation and shake flask culture of strains. Preservation number: CCTCC M2011423 Bacillus licheniformis BFT-4, preservation number: CCTCC M2011425 Pseudomonas stutzeri BRH-6, preservation number: CCTCC M2011427 Pseudomonas putida BRZ-1, were inoculated on beef extract peptone slope solid On the culture medium, cultivate it at 30°C for 24 hours to activate it, then insert it into a shaker flask containing 100ml of beef extract peptone liquid medium, and cultivate it for 24 hours at 30°C in a shaker at 150 rpm, and set aside;

[0036] Seed tank fermentation: put the beef extract peptone liquid medium into the seed tank, steam high-temperature sterilization and cooling in the seed tank, and insert the shake flask bacteria in step (1) according to the inoculation amount of 5%; start the mixer The speed is 180 rpm, the temperature is 30°C, and the ventilation volume is 4m 3 / h, tank pressure 0.1M Pa, cultivated for 24 hours under the condition of natural pH, and set...

Embodiment 2

[0042] Slant activation and shake flask culture of strains. The specific implementation is the same as step 1 of embodiment 1);

[0043] Seed tank fermentation: put the beef extract peptone liquid medium into the seed tank, steam high-temperature sterilization and cooling in the seed tank, and insert the shake flask bacteria in step (1) according to the inoculation amount of 8%; start the mixer The speed is 180 rpm, the temperature is 30°C, and the ventilation volume is 4m 3 / h, tank pressure 0.1M Pa, cultivated for 24 hours under the condition of natural pH, and set aside;

[0044] Preparation of bulk products:

[0045] Bacillus licheniformis BFT-4 adopts solid fermentation, and the medium of solid fermentation is bran 85%, rice bran: 10%, glucose 3%, K 2 HPO 4 2%, D biotin 0.05%, add water according to the ratio of material to water 1:1; sterilize the solid medium and cool it down, weigh the liquid strain of the seed tank in step (2) according to the proportion of 3% of ...

Embodiment 3

[0049] Slant activation and shake flask culture of strains. The specific implementation is the same as step 1 of embodiment 1);

[0050] Seed tank fermentation: put the beef extract peptone liquid medium into the seed tank, steam high-temperature sterilization and cooling in the seed tank, and insert the shake flask bacteria in step (1) according to the inoculation amount of 10%; start the mixer The speed is 180 rpm, the temperature is 30°C, and the ventilation volume is 4m 3 / h, tank pressure 0.1M Pa, cultivated for 24 hours under the condition of natural pH, and set aside;

[0051] Preparation of bulk products:

[0052] Bacillus licheniformis BFT-4 adopts solid fermentation, and the medium of solid fermentation is bran 90%, rice bran: 5%, glucose 3%, K 2 HPO 4 2%, D biotin 0.05%, add water according to the ratio of material to water 1:1; sterilize the solid medium and cool it down, weigh the liquid bacteria in the seed tank of step (2) according to the proportion of 4% o...

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Abstract

The invention provides a microbe-enzyme composite preparation used for restoring water in an urban and rural polluted river, and its preparation method. The urban and rural polluted river restoration microbe-enzyme composite preparation is characterized in that the microbe mainly comprises 30-60% of Bacillus licheniformis, 25-35% of a nitrobacterium, and 15-35% of pseudomonas putida; and the composite enzyme mainly comprises 40-60% of a protease, 15-25% of an amylase and 25-35% of a cellulase. The optimized microbe is compounded with the composite enzyme to prepare the microbe-enzyme composite preparation against water of different pollution degrees, the addition amount of the microbe is 1-5g / m<3>, and the addition amount of the composite enzyme is 0.01-0.05g / m<3>. The microbe-enzyme composite preparation is added to the urban and rural polluted river to carry out microbial reinforced restoration with aeration as an auxiliary measure, can effectively improve defects of low microbe metabolism activity, low environmental tolerance, short retention time in the polluted water and the like existing in the prior art, and has a substantial water purifying effect.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a microorganism-enzyme composite preparation capable of efficiently degrading ammonia nitrogen and COD in urban and rural polluted river waters and restoring the river environment, and a preparation method thereof. Background technique [0002] With the rapid development of my country's economy, my country's water environment problems have become increasingly prominent, and urban and rural water environments have encountered unprecedented threats. Surveys have shown that more than 90% of the rural water environment in the Yangtze River Delta region has lost its basic functions. Urban and rural rivers have been seriously degraded due to pollution and ecosystems. Nutritization, the main pollution index is potassium permanganate index (COD Mn ) and ammonia nitrogen, in addition, most of the river water turned black and smelly, and the pathogenic bacteri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12N1/20C12N9/50C12N9/42C12N9/26C12R1/10C12R1/38C12R1/40
CPCY02A20/402
Inventor 汤江武王新姚晓红吴逸飞周莉谢尚侃
Owner 浙江绿凯环保科技股份有限公司
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