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Extraction method of total alkaloids from tripterygium hypoglaucum and application of extract

An extraction method and a technology of total alkaloids, which are applied in the field of plant extraction technology and drug research, can solve the problems of long soaking time, many impurities of total alkaloids, and difficult separation and purification

Inactive Publication Date: 2012-04-11
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional extraction method of Total alkaloids of THH (THHta) in Kunming Mountains takes too long to soak the medicine (add organic solvent for up to 6 days, as shown in the literature [Ao Lin, Cao Jia, Xu Ying, etc., Kunming Mountain Observation on the apoptosis of HL60 cells induced by total alkaloids of Begonia. Journal of Third Military Medical University. 2001, 23(11): 1273-1275]) and the obtained total alkaloids have more impurities and are not easy to separate and purify

Method used

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  • Extraction method of total alkaloids from tripterygium hypoglaucum and application of extract
  • Extraction method of total alkaloids from tripterygium hypoglaucum and application of extract
  • Extraction method of total alkaloids from tripterygium hypoglaucum and application of extract

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Effect test

Embodiment 1

[0048] Take 2kg of Kunming Begonia peeled root, crush it and pass through a 10-mesh sieve, soak it with 10 times the amount of 61% ethanol and place it in an ultrasonic instrument for 3 hours, filter out the medicinal solution, add 10 times the amount of 61% ethanol twice, soak At the same time, they were respectively placed in an ultrasonic instrument for 2 hours and 1 hour, and the medicinal liquid was filtered out, combined with the first medicinal liquid, and concentrated. Add 30% HCl to adjust the pH to 2-3, extract 3 times with an equivalent amount of chloroform, and keep the aqueous phase. Join Na 2 CO3 , adjust the pH to 9-11, then extract 3 times with an equivalent amount of chloroform, and combine the extracts to recover chloroform by rotary evaporation at 40-50°C to obtain 19.8 g of total alkaloids;

Embodiment 2

[0050] Take 100g of Kunming Begonia peeled root, crush it and pass through a 10-mesh sieve, soak it with 10 times the amount of 69% ethanol and place it in an ultrasonic instrument for 3 hours, filter out the medicinal solution, add 10 times the amount of 69% ethanol twice, soak At the same time, they were respectively placed in an ultrasonic instrument for 2 hours and 1 hour, and the medicinal liquid was filtered out, combined with the first medicinal liquid, and concentrated. Add 45% HCl to adjust the pH to 2-3, extract 3 times with an equivalent amount of chloroform, and keep the aqueous phase. Add Na 2 CO 3 , adjust the pH to 9-11, pass the alkaline solution through a C18 solid-phase extraction device, rinse the solid-phase extraction device with water for 30 minutes, and rinse with 45% ethanol. After collecting the rinse solution, recover ethanol by rotary evaporation to obtain 0.97g of total alkaloids ;

Embodiment 3

[0052] Take 100g of Kunming Begonia peeled root, crush it and pass through a 10-mesh sieve, soak it with 10 times the amount of 65% ethanol and place it in an ultrasonic instrument for 3 hours, filter out the medicinal solution, add 10 times the amount of 65% ethanol twice, soak At the same time, they were respectively placed in an ultrasonic instrument for 2 hours and 1 hour, and the medicinal liquid was filtered out, combined with the first medicinal liquid, and concentrated. Add 40% HCl to adjust the pH to 2-3, extract 3 times with an appropriate amount of chloroform, and keep the aqueous phase. Join Na 2 CO 3 , adjust the pH to 9-11, pass the alkaline solution through a C18 solid-phase extraction device, rinse the solid-phase extraction device with water for 30 minutes, and rinse with 50% ethanol. After collecting the washing liquid, recover ethanol by rotary evaporation to obtain 0.95 g of total alkaloids .

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Abstract

The invention discloses an extraction method of total alkaloids from tripterygium hypoglaucum and an application of the extract. The extraction method comprises the following steps of: taking a certain amount of tripterygium hypoglaucum, pulverizing after peeling and removing the root, adding a certain amount of ethanol solution of a finite concentration for performing multiple extraction assisted by ultrasonic equipment, combining the extracted solutions and concentrating, adding concentrated hydrochloric acid of a finite concentration to the concentrated solution for regulating the pH value, filtering after fully mixing for a period of time, extracting the filtrate by chloroform many times, reserving the raffinate phase, regulating the pH value of the raffinate phase to be alkaline, extracting the alkaline solution by chloroform many times, combining the chloroform phases, and evaporating for recovering the chloroform to obtain total alkaloids, or performing solid phase extraction on the alkaline solution through C18 columns to obtain total alkaloids. The extract obtained according to the invention can be used for treating tumor and autoimmune disease.

Description

technical field [0001] The invention belongs to the field of plant extraction technology and drug research, and specifically relates to a method for extracting total alkaloids from Kunming mountain crabapple plants and an application of the extract obtained by the extraction method. Background technique [0002] Cancer poses a major threat to human health and survival, and anti-tumor drugs are still one of the most important means of cancer treatment at present and for a long time to come. Most of the existing tumor chemotherapy drugs have the disadvantages of low selectivity and high toxicity. Discovering new antitumor drugs with high selectivity, low toxicity and strong specificity has become an important direction in the research and development of anticancer drugs. my country has the largest population in the world and a high incidence of tumors. The development of new anti-tumor drugs is also the focus of my country's new drug research and development. [0003] The di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P35/00A61P37/02A61K125/00
Inventor 刘晋祎曹佳敖琳杨录军黄晓春姜晓
Owner ARMY MEDICAL UNIV
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