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Method for determining effective component content in chenopodium ambrosioides volatile oil

A technology for the volatile oil and active ingredients of Nepeta nepeta, which is applied in the directions of measuring devices, material separation, and analysis of materials, etc., can solve the problems of unreported content determination methods, and achieve the effect of avoiding unstable measurement results

Active Publication Date: 2012-05-30
TIANJIN TASLY PHARMA CO LTD
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  • Claims
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Problems solved by technology

According to literature reports, ascaridin, p-cymene and α-terpinene are the main components of the volatile oil of Nepeta nepeta, and they are representative to a certain extent, but the method for simultaneous determination of these three components has not been reported
Volatile oil components are generally selected for analysis by gas chromatography, but gas chromatography adopts capillary columns to affect the stability of its measurement due to factors such as shunting. The invention provides a method for simultaneously measuring ascaridin and paracystin in the volatile oil of Nepeta nepeta with HPLC. HPLC Determination Method of Anthracene and α-Terpinene

Method used

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  • Method for determining effective component content in chenopodium ambrosioides volatile oil
  • Method for determining effective component content in chenopodium ambrosioides volatile oil
  • Method for determining effective component content in chenopodium ambrosioides volatile oil

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Embodiment 1

[0054] 1. Apparatus and materials

[0055] American Agilent 1100 high performance liquid chromatograph (UV detector, binary pump, autosampler, column thermostat).

[0056] Ascarisin reference substance (self-made, the structure is confirmed to be ascarisin, the purity is 90.0% by HPLC analysis), the paracymene reference substance (St.Louis, USA, purity is 99.0%), α-terpinene control Product (self-made, the structure is confirmed to be α-terpinene, the purity is 92.37% by HPLC), two pure water (self-made), acetonitrile (Merck) are chromatographically pure, and other reagents are analytically pure.

[0057] Nepeta sinensis (identified as Chenopodium ambrosioides L. by Professor Wang Zhenyue, Department of Chinese Medicine Resources and Development, School of Pharmacy, Heilongjiang University of Traditional Chinese Medicine) volatile oil was provided by Shaanxi Tasly Plant Pharmaceutical Co., Ltd.

[0058] 2. Methods and results

[0059] 2.1 Chromatographic conditions

[0060] Chromatograp...

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Abstract

The invention relates to a method for determining effective component contents in chenopodium ambrosioides volatile oil, and the method comprises the following steps: step 1, preparing a chenopodium ambrosioides volatile oil solution; step 2, injecting into a high performance liquid chromatograph for peak area determination; step 3, calculating the effective component contents in the chenopodium ambrosioides volatile oil.

Description

Technical field [0001] The invention relates to a method for the content of effective ingredients in traditional Chinese medicine, in particular to a method for determining the content of effective ingredients in the volatile oil of Nepeta sinensis. Background technique [0002] Nepeta is a plant of Chenopodiaceae Chenopodium ambrosioides L. The whole plant with ears, has the effects of expelling wind, killing insects, regulating menstruation and relieving pain. It is used for skin rheumatic arthralgia, dysmenorrhea, amenorrhea, skin eczema and snake insects. bite. According to literature reports, ascarisin, p-cymene and α-terpinene are the main components of the volatile oil of Nepeta sativus and have a certain representativeness, but there has been no report on the method of determining the content of these three components at the same time. . Volatile oil components are generally analyzed by gas chromatography, but gas chromatography adopts capillary column due to factors su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 张兰兰周水平黄芝娟李瑞明宋兆辉
Owner TIANJIN TASLY PHARMA CO LTD
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