Breeding method of low-salt-resistant line of blue crab

A technology of Portunus trituberculatus and strains, which can be used in fish farming, application, climate change adaptation, etc., can solve the problems of low survival rate, poor stress resistance, and quality degradation of cultured species, and achieve simple and convenient operation and stress resistance Strong, growth speed and the effect of raising the survival rate of breeding

Active Publication Date: 2012-06-20
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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AI Technical Summary

Problems solved by technology

The breeding of new varieties of Portunus trituberculatus started in 2005, and the work was mainly carried out for growth and disease resistance traits, but there is no report on the breeding method of Portunus trituberculatus strains resistant to low salt
Therefore, it is of great significance to cultivate new varieties of cultured crabs with low salt tolerance to solve the problems of quality degradation, poor stress resistance, and low survival rate of cultured species.

Method used

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Effect test

Embodiment 1

[0018] The breeding method of Portunus trituberculatus low-salt strain of the present invention comprises the following steps:

[0019] 1. In September 2005, four different populations of Portunus trituberculatus were collected from the Yalu River Estuary, Laizhou Bay, Haizhou Bay and Zhoushan, and the "Huangxuan No. 1" fast-growing Portunus trituberculatus species was bred through group selection as a basis group.

[0020] 2. At the end of July 2009, according to the requirement of 5% selection intensity and 1:1 male-to-female ratio, 1,000 healthy juvenile crabs growing to 100 days old were selected from the basic population of "Huangxuan No. 1" portunus trituberculatus artificially bred with excellent growth characteristics. Only low-salinity stress was applied. The salinity stress was carried out in the indoor cement pond, and the stress salinity was controlled at the median lethal concentration of 11‰, and the stress was continued for 72 hours. 452 surviving individuals w...

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Abstract

The invention provides a breeding method of a low-salt-resistant line of blue crab. The breeding method comprises the steps of: based on blue crab which is artificially bred for multiple years and has excellent growth characters as a base colony, subjecting young crab of 100 days old to low-salinity stress experiment, and carrying out two generations of low-salinity stress breeding to screen low-salt-resistant line of blue crab, wherein the screened core breeding colony can be used for production and application after passage propagation. According to the invention, a quick growth colony is selected as the base colony, and the low-salt-resistant stress-resistant characters are aggregated in the blue crab base colony through low-salt-resistant breeding, thus the cultured new line has strong stress resistance, and is suitable for breeding in low-salinity areas, and the growth rate and breeding survival rate are significantly increased; and the method is simple and convenient to operate, and is applicable to breeding of the low-salt-resistant line of blue crab.

Description

technical field [0001] The invention relates to the field of aquatic genetics and breeding, in particular to a method for breeding low-salt-tolerant strains of Portunus trituberculatus. Background technique [0002] Portunus trituberculatus is one of the important seawater crustacean species in my country. It has high economic value and huge market demand. Artificial breeding began in the late 1980s along the coast of my country. In recent years, the breeding industry of Portunus trituberculatus in my country has developed rapidly, with a breeding area of ​​500,000 mu and an annual output of 100,000 tons. Breeding of improved species is one of the important keys to the sustainable and stable development of Portunus aquaculture. The cultivation of superior species with strong stress resistance through artificial directional selection can enhance the ability of crabs to resist environmental stress and improve the survival rate of breeding. [0003] At the beginning of this ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 李健刘萍高保全
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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