32 results about "Salinity stress" patented technology

The invention belongs to the technical field of shrimp feed, and particularly relates to a special insect-derived health-care feed for shrimp fry. The health-care feed comprises the following components in parts by weight: 15-20% of black soldier fly dry powder, 5-10% of fish meal, 15-25% of peanut meal, 10-28% of flour, 2-5% of mixed oil, 5-15% of soybean powder and 10-25% of corn flour. The special health-care feed for shrimp fry can enhance the anti-stress capability of shrimp fry, improve the growth speed and improve the survival rate. The growth experiment of penaeus vannamei fry shows that the health-care feed not only can promote the growth of shrimp fry, but also can improve the survival rate of the shrimp fry. The salinity stress experiment, the temperature stress experiment and the desalination standard thickening experiment of the penaeus vannamei fry show that the health-care feed can improve the stress capacity of the penaeus vannamei fry on salinity and temperature change, thereby improving the survival rate of the fry in the desalination standard thickening stage and improving the economic benefit of shrimp fry culture.

A salt-resistant preparation for improving the salt resistance of the wheat and an application method thereof belong to the technical field of improving the salt resistance of the wheat to increase the wheat production. The method comprises the following steps: 1) soaking in the salt resistance preparation, wherein, with the overall weight being 1kg , the final composite ingredients are 3-4g of Ca (NO3)2, 1.2-1.5g of KH2PO4, 1.5-2g of KI, 0.35g of MnSO4, 10mg of Na2SeO3, 40-60mg of cytokinin, and water in balancing amount; 2) spraying the salt resistant preparation on the surfaces of leaves, wherein, with the overall weight being 1kg, the final composite ingredients are 1-1.2g of Ca (NO3)2, 0.25-0.3g of KH2PO4, 0.35g of KI, 0.1g of MnSO4, 2mg of Na2SeO3, 2-10mg of cytokinin, and water in balancing amount. Wheat seeds are soaked in the salt resistant preparation for one time before being planted, and the salt resistant preparation is sprayed on the surfaces of the leaves after the fifteen days of wheat seedlings turn green in the spring of the next year. The invention has the advantages of significantly improving the ratio of emergence of wheat seeds under high salinity, promoting the growing development of wheat plants, increasing the production of the wheat by 15-20 percent, and being suitable for the soil with salt contents being 0.3 percent.

The invention provides a high-efficiency portunus trituberculatus seedling conveying method, and belongs to the technical field of cultivation of portunus trituberculatus. The high-efficiency portunus trituberculatus seedling conveying method comprises the following steps of (1) seedling selection: randomly selecting a first stage of juvenile portunus trituberculatus from a nursery pond by using 72-hour low-salt medial lethal concentration 10.79 of the first stage of the juvenile portunus trituberculatus as dosage, performing a salinity stress experiment, calculating the survival rate after 72 hours, and selecting a second stage of juvenile portunus trituberculatus in a nursery pond with the survival rate higher than 50% as conveying seedlings; and (2) a conveying method: uniformly mixing wheat shells which are soaked and then are drained off and the second stage of the juvenile portunus trituberculatus according to the weight ratio of 1:1 at first, placing the mixture in a seedling bag, inflating oxygen in the seedling bag, binding the opening of the bag by using a rubber band, placing the seedling bag in a bubble chamber, finally, placing biological ice bags in the bubble chamber, and sealing the bubble chamber by using adhesive tapes. By the high-efficiency portunus trituberculatus seedling conveying method, the seedling conveying time can be 10-12 hours, the conveying yield reaches 98.5%, the damage rate of the juvenile portunus trituberculatus reaches 1.2%, and the seedling cultivation survival rate is guaranteed.

The invention belongs to the field of feeds and relates to a preparation method of a compound feed specially used for aquacultural breeding of prawns. The invention provides a preparation method of a feed especially for high-salinity breeding of penaeus vannamei. The feed can satisfy demand on nutrition for the penaeus vannamei under high salinity (higher than 3%), is good in stability in water, has strong anti-stress capability, is high in survival rate and accelerates growth, is low in feed coefficient, and is economical and practical, thereby solving the problems of slow growth, high diseases incidence and low survival rate of the penaeus vannamei under high salinity stress breeding. The feed can promote healthy development of the aquacultural breeding of prawns.

The invention provides methods and constructs for enhancing the expression of nucleotide sequences encoding proteins that comprise at least a DnaJ-type J-domain to provide tolerance to plants or plantcells against abiotic stress, especially dehydration and salinity stress.

The invention provides an immersed dunaliella salina liquid ultrafiltration system. The system comprises a track tank, an immersed ultrafiltration assembly, a concentration tank, a water producing system, an aeration system and a backwashing system. By means of a dunaliella salina liquid concentration method of the ultrafiltration system, dunaliella salina heavily proliferated under the approximate optimum growth salinity condition is effectively concentrated, the size of low-salinity dunaliella salina liquids is greatly reduced while the biomass is kept, high brine amount added to realize thehigh-salinity stress growth condition later is reduced, the death rate of dunaliella salina in the concentration process can be reduced to the greatest extent through precise control on ultrafiltration work pressure, and more effective biomass can be obtained.

The invention discloses a low-fish-meal compound feed for litopenaeus vannamei suitable for low-salinity culture conditions. The feed is prepared from, by weight, 60-75 parts of protein-sourced raw material, 3-6 parts of fat-sourced raw material, 22-28 parts of sugar-sourced raw material, 0.3-1 part of anti-salinity stress substance, 0.05-0.3 part of immunopotentiator, 0.2-1 part of essential amino acid, 0.5-2 parts of compound vitamin, 0.25-0.45 part of monovitamin, 0.5-2 parts of compound mineral salt, 0.01-0.05 part of an enzyme preparation, 1-2 parts of a phosphorus raw material and 0.9-1.1 parts of a feed adhesive. According to the feed, the content of fish meal is reduced to 14-16 wt% by balancing the composition of amino acids, and the salinity-resistant stress substance and immunopotentiator are added to improve the culture effect under low salinity. The litopenaeus vannamei fed with the low-fish-meal compound feed is high in growth speed, can utilize the feed well, has high survival rate, good oxidation resistance and high salinity stress resistance under the low-salinity culture condition, and the culture effect basically the same as that of common high-fish-meal feed canbe achieved.

The invention discloses a method for screening sinonovacula constricta with strong salt tolerance by utilizing osmotic pressure. The method comprises the following steps: culturing sinonovacula constricta in a same culture environment, performing grouping salinity stress treatment, determining osmotic pressure and carrying out final screening. By screening the sinonovacula constricta through the method, the sinonovacula constricta with good salt resistance can be effectively screened out, and the method is simple, free of complex equipment, high in screening efficiency and high in commercial value.

The invention discloses a western balsam poplar PtrZFP103 gene as well as a coding protein and application thereof, and belongs to the field of biotechnology. A nucleotide sequence of the western balsam poplar PtrZFP103 gene is shown as SEQ ID No: 1. An amino acid sequence of the coding protein of the western balsam poplar PtrZFP103 gene is shown as SEQ ID No: 2. The application is to improve the anti-adversity ability of a plant under high salinity stress. A full-length sequence of a coding region of the western balsam poplar PtrZFP103 gene is 507bp, and 168 amino acids are coded. The damage extent of transgenic line cells is discovered to be obviously lower than that of WT under the high salinity stress, and physiological index results also verify that the anti-adversity ability of an overexpression line of the PtrZFP103 gene is obviously higher than that of the WT. As the PtrZFP103 is discovered to specifically improves the resistance of the plant under the high salinity stress, a high salinity-resistant new variety of a PtrZFP103 transgene can be cultivated, and important significance is supplied for forest tree molecular breeding on the aspect of cultivating the new variety.

The invention relates to the technical field of irrigation, in particular to a crop irrigation method based on salinity elution rate, which is characterized in that the salinity elution rate of crops is calculated by combining the influence of salinity stress, water quality and the like on the irrigation amount of the crops, and the salinity elution rate of the crops is calculated by monitoring the moisture and salinity of the soil depth corresponding to the root systems of the crops. The irrigation device is controlled to conduct irrigation and drainage operation, redundant salt of crop roots is removed, the stress effect of the salt on crop growth is reduced, the crop yield is guaranteed, efficient utilization of existing water resources is achieved, and meanwhile waste of the water resources is reduced.

The invention discloses a judgment method for viability of juvenile eriocheir sinensis, and relates to the field of river crab culture in the modern agricultural industry. According to the judgment method for the viability of the juvenile eriocheir sinensis, the viability of the juvenile eriocheir sinensis is judged by analyzing the stress expression and osmotic pressure regulation condition of the eriocheir sinensis under salinity stress. The judgment method for the viability of the juvenile eriocheir sinensis established by the invention has extremely good reliability, the breeding resources from the juvenile crab to the adult crab stage are saved, and meanwhile, the survival of the superior and the inferior is carried out according to the judgment result of the viability, so that the product quality of the adult crab after the eriocheir sinensis is fattened can be effectively improved.