Western balsam poplar PtrZFP103 gene as well as coding protein and application thereof
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A protein-encoding and gene-encoding technology, applied in Populus trichocarpa PtrZFP103 gene and its encoded protein and its application fields, can solve problems such as difficulty in satisfying directional cultivation of new trees, long growth cycle, and long time for new stress-resistant varieties
Inactive Publication Date: 2017-02-15
NORTHEAST FORESTRY UNIVERSITY
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Problems solved by technology
Traditional hybrid breeding requires fine varieties as the main resource. Due to the long growth cycle of forest trees and unclear genetic mechanism, it takes a long time to obtain new stress-resistant varieties and the efficiency is very low. It is difficult to meet the requirements of directional cultivation of new tree products for different purposes.
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specific Embodiment approach 1
[0031] Embodiment 1: The nucleotide sequence of PtrZFP103 gene of Populus trichocarpa in this embodiment is shown in SEQ ID No: 1.
[0032] The full-length sequence of the coding region of the Populus trichocarpa PtrZFP103 gene in this embodiment is 507 bp.
specific Embodiment approach 2
[0033] Embodiment 2: The amino acid sequence of the protein encoded by PtrZFP103 gene of Populus trichocarpa in this embodiment is shown in SEQ ID No: 2.
[0034] In this embodiment, the encoded protein of Populus trichocarpa PtrZFP103 gene consists of 168 amino acids.
specific Embodiment approach 3
[0035] Specific Embodiment Three: The application of the Populus trichocarpa PtrZFP103 gene in this embodiment refers to the application in improving the stress resistance ability of plants under high-salt stress.
[0036] The following examples are conventional methods unless otherwise specified. The materials, reagents, enzymes, competent cells and plasmids used can all be obtained from commercial sources unless otherwise specified.
[0037] 1. The acquisition and expression characteristics analysis of PtZFP103 gene in Populus trichocarpa is as follows:
[0038] 1.1 Cloning of the target gene
[0039] 1.1.1 Obtaining the target gene sequence
[0040] Design primers at both ends of the target gene sequence as shown in Table 1.
[0041] Table 1 The sequences of primers used for the cloning of the target gene according to the present invention
[0042]
[0043] The total RNA of Populus trichocarpa (Torr. & Gray) female clone Nisqually1 was extracted by CTAB method, such ...
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Abstract
The invention discloses a western balsam poplar PtrZFP103 gene as well as a coding protein and application thereof, and belongs to the field of biotechnology. A nucleotide sequence of the western balsam poplar PtrZFP103 gene is shown as SEQ ID No: 1. An amino acid sequence of the coding protein of the western balsam poplar PtrZFP103 gene is shown as SEQ ID No: 2. The application is to improve the anti-adversity ability of a plant under high salinity stress. A full-length sequence of a coding region of the western balsam poplar PtrZFP103 gene is 507bp, and 168 amino acids are coded. The damage extent of transgenic line cells is discovered to be obviously lower than that of WT under the high salinity stress, and physiological index results also verify that the anti-adversity ability of an overexpression line of the PtrZFP103 gene is obviously higher than that of the WT. As the PtrZFP103 is discovered to specifically improves the resistance of the plant under the high salinity stress, a high salinity-resistant new variety of a PtrZFP103 transgene can be cultivated, and important significance is supplied for forest tree molecular breeding on the aspect of cultivating the new variety.
Description
technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the PtrZFP103 gene of Populus trichocarpa and its encoded protein and application. Background technique [0002] Forests provide social, ecological and economic benefits, but as the climate continues to change trees experience stresses such as drought, high salinity and heat which cause them to be disturbed and die. Environmental changes such as temperature, insects, pathogens, and high salinity are predicted to be major contributors to widespread forest die-offs. Therefore, cultivating salt-resistant tree species is of great significance for improving forest structure. Traditional hybrid breeding requires fine varieties as the main resource. Due to the long growth cycle of forest trees and unclear genetic mechanism, it takes a long time to obtain new stress-resistant varieties and the efficiency is very low. It is difficult to meet the requirements of directional breedin...
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