New application of Arabidopsis at4g09820 gene in plant salt tolerance

A technology of at4g09820, Arabidopsis thaliana, applied in the fields of botanical equipment and methods, applications, plant products, etc., can solve problems such as no related research and reports

Active Publication Date: 2016-02-17
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are no relevant studies and reports on other uses of this gene

Method used

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  • New application of Arabidopsis at4g09820 gene in plant salt tolerance
  • New application of Arabidopsis at4g09820 gene in plant salt tolerance
  • New application of Arabidopsis at4g09820 gene in plant salt tolerance

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Embodiment 1

[0020] In order to prove that the At4g09820 gene is related to the salt resistance of plants, the inventors conducted comparative analysis experiments.

[0021] the following tt8-1 The mutant is the T-DNA insertion mutant (CS111) of the At4g09820 gene obtained from the Arabidopsis Resource Center. The At4g09820 gene of this mutant is inactivated and cannot correctly encode related proteins. Further verification shows that the At4g09820 gene is related to the plant's salt resistance function .

[0022] The specific experimental verification process is as follows:

[0023] (1) Verify that the At4g09820 gene specifically regulates the ion toxicity response caused by NaCl

[0024] Arabidopsis wild-type (WT) and tt8-1 Mutant seeds were sown on 0.6% agar MS medium and MS medium containing 100 mM NaCl or 200 mM mannitol. The growth environment is: the light / dark cycle is 8 / 16h, the temperature is 22°C, the dark temperature is 18°C, the relative humidity is 70%, and the light ...

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Abstract

The invention belongs to the technical field of genetic engineering application, and in particular relates to the application of Arabidopsis thaliana At4g09820 gene in cultivating new varieties of salt-tolerant plants. Arabidopsis At4g09820 gene, also known as TT8 The gene, whose CDS length is 1557bp, encodes a protein of 518 amino acids, belongs to the bHLH (basic? helix-loop-helix) type transcription factor, and regulates the biosynthesis of proanthocyanidins and anthocyanins in the flavonoid synthesis pathway. Through the research of the At4g09820 gene in high-salinity stress, the inventor found that the gene specifically regulates the ion poisoning response caused by NaCl ， It shows good salt-resistance characteristics, so it has a relatively important application in plant salt-resistance. Through further research and transformation of the gene, excellent salt-tolerant plant varieties can be bred.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering application, and in particular relates to the application of Arabidopsis thaliana At4g09820 gene in cultivating new varieties of salt-tolerant plants. Background technique [0002] At present, the shortage of global water resources is increasing day by day, and the extensive use of chemical fertilizers by humans has caused soil salinization to become increasingly serious, restricting the sustainable production and development of agriculture. With the deepening of molecular biology and genetic engineering research, many scientific and technological workers in the world are also trying to find genes related to plant salt resistance. They plan to improve the salt resistance of crops through genetic engineering technology, but there are very few achievements available at present. . [0003] Arabidopsis ( Arabidopsisthaliana ) belongs to the Brassicaceae Arabidopsis plant, also known as A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82A01H5/00
Inventor 张骁赵翔王琳丹王进
Owner HENAN UNIVERSITY
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