Method for screening sinonovacula constricta with strong salt tolerance by utilizing osmotic pressure

A technology of osmotic pressure and razor clam, which is applied in the field of breeding new varieties of razor clam, can solve the problems of low breeding efficiency and poor effect, and achieve the effects of simple operation, strong salt tolerance, and rapid environmental adaptability

Pending Publication Date: 2020-10-30
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for using osmotic pressure to screen razor clams with strong salt tolerance ability, so as to solve the problems of low breeding efficiency and poor effect in the cultivation of new varieties of razor clams

Method used

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  • Method for screening sinonovacula constricta with strong salt tolerance by utilizing osmotic pressure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The present invention utilizes osmotic pressure to screen the method for strong salt-tolerant ability of razor clams, comprising

[0029] (1) Preprocessing:

[0030] Take healthy adult razor clams with no damage on the outside, and keep them in a recirculating aquaculture aquarium for a week. The salinity of the water body is 20.47, the pH is 8.1, and the water temperature is 25.12°C. Continuously aerate them to adapt to the environment. Feed the mixed algae of Chrysophylla and Chaetoceros 2 times, and the amount of input is 5% of the mass of razor clams;

[0031] (2) Group salinity stress treatment:

[0032] 5 experimental groups of 5, 15, 20, 25, 35 water body salinity are set, and 50 constricted razor clams processed by step (1) are put into each group, and the salinity stress experiment is carried out;

[0033] (3) Determination of osmotic pressure

[0034] At 0, 0.5, 1, 1.5, 2, 4, 8, 12, 24, 48, 72, and 96 hours after the experimental stress, the razor clams in ...

Embodiment 2

[0038] The present invention utilizes osmotic pressure to screen the method for strong salt-tolerant ability of razor clams, comprising

[0039] (1) Preprocessing:

[0040] Take healthy adult razor clams with no damage on the outside, and keep them in a recirculating aquaculture aquarium for a week. The salinity of the water body is 19.53, the pH is 7.9, and the water temperature is 21.26°C. Continuously inflate them to adapt to the environment. Feed the mixed algae of Chrysophylla and Chaetoceros 2 times, and the amount of input is 5% of the mass of razor clams;

[0041] (2) Group salinity stress treatment:

[0042] 5 experimental groups of 5, 15, 20, 25, 35 water body salinity are set, and 50 constricted razor clams processed by step (1) are put into each group, and the salinity stress experiment is carried out;

[0043] (3) Determination of osmotic pressure

[0044] At 0, 0.5, 1, 1.5, 2, 4, 8, 12, 24, 48, 72, and 96 hours after the experimental stress, the razor clams in...

Embodiment 3

[0048] The present invention utilizes osmotic pressure to screen the method for strong salt-tolerant ability of razor clams, comprising

[0049] (1) Preprocessing:

[0050] Take healthy and undamaged adult razor clam shells and keep them in a recirculating aquaculture aquarium for a week. The salinity of the water body is 21.41, the pH is 8.3, and the water temperature is 28.98°C. Continuously inflate them to adapt to the environment. Feed the mixed algae of Chrysophylla and Chaetoceros 2 times, and the amount of input is 5% of the mass of razor clams;

[0051] (2) Group salinity stress treatment:

[0052] 5 experimental groups of 5, 15, 20, 25, 35 water body salinity are set, and 50 constricted razor clams processed by step (1) are put into each group, and the salinity stress experiment is carried out;

[0053] (3) Determination of osmotic pressure

[0054] At 0, 0.5, 1, 1.5, 2, 4, 8, 12, 24, 48, 72, and 96 hours after the experimental stress, the razor clams in each group...

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Abstract

The invention discloses a method for screening sinonovacula constricta with strong salt tolerance by utilizing osmotic pressure. The method comprises the following steps: culturing sinonovacula constricta in a same culture environment, performing grouping salinity stress treatment, determining osmotic pressure and carrying out final screening. By screening the sinonovacula constricta through the method, the sinonovacula constricta with good salt resistance can be effectively screened out, and the method is simple, free of complex equipment, high in screening efficiency and high in commercial value.

Description

technical field [0001] The invention belongs to the technical field of cultivating new varieties of razor clams, and in particular relates to a method for screening razor clams with strong salt tolerance by using osmotic pressure. Background technique [0002] Razor razor clam, commonly known as "razor clam", is a bivalve clam shellfish that lives in the ocean and is a common seafood ingredient. The shell is brittle and thin, oblong and square. From the top of the shell to the belly edge, there is a slanting groove, hence the name razor clam. It likes to live in soft mud flats in the inner bay, and is a common mollusk in the coastal areas of our country. Razor razor clams belong to the phylum Molluscs, Class Phyllobranchia, Class Heterodontica, Order Clamidae, Shellfish of the family Clamidae, and are one of the four major cultured shellfish in my country. Widely distributed in my country and the coast of Japan, mainly distributed in the north and south coastal beaches in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/54A01K61/90
CPCA01K61/54A01K61/90Y02A40/81
Inventor 徐娴何琳董迎辉何京林志华
Owner ZHEJIANG WANLI UNIV
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