Composite amplification system and detection kit of mouse short tandem repeat
A compound amplification system and short tandem repeat technology, applied in the biological field, can solve the problem that STR typing has not been widely used
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[0106] The following is our application of the present invention to 15 cases of mouse cells CTLL-2, 3T6 Swiss, NS-1, P3X63Ag8.65, J774A.1, LM(TK-), WEHI-13VAR, P388, SP2 / 0, SC-1 , BALB / C 3T3Clone A31, M.dunni, NIH / 3T3, A9 and 3T3 Swiss DNA samples for the specific implementation of testing. The samples are all common cells and are commercially available. Our company also keeps them and can be publicly distributed.
[0107] 1. DNA extraction
[0108] Tissue DNA extraction kit (Tiangen Biochemical) was used to analyze the cultured CTLL-2, 3T6 Swiss, NS-1, P3X63Ag8.65, J774A.1, LM (TK-), WEHI-13VAR, P388, SP2 / 0, SC -1. BALB / C 3T3Clone A31, M. dunni, NIH / 3T3, A9 and 3T3 Swiss cells were subjected to DNA extraction. The operation steps were in accordance with the instructions. After DNA extraction, the DNA was quantified with an ultraviolet spectrophotometer and diluted to 2ng / μl.
[0109] 2. PCR
[0110] 2.1 Reaction system:
[0111] A total of 8 pairs of primers at Jarid1 site and 7 STR...
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