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Iron-rich black fungus and production method thereof

A black fungus, iron-rich technology, applied in the fields of botanical equipment and methods, fertilizer mixtures, gardening, etc., can solve problems such as the development of iron-rich agaric systems without precedents

Active Publication Date: 2012-08-15
MICROHARBOR BIOTECH JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, so far, there is no precedent for the development of iron-rich fungus systems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: 3 L containing iron (FeSO 4 ·7H 2 O) Submerged fermentation of Xinke No. 5 liquid at 2100 mg / L

[0034] 1) Insert Xinke No. 5 purchased from Zhejiang Qiming Edible Mushroom Co., Ltd. into the sterilized PDA comprehensive medium for in vitro culture;

[0035] 2) The test tube strains were inserted into the PDA comprehensive culture medium with an iron content of 100 mg / L for cultivation, and iron-resistant strains were screened out;

[0036] 3) with FeSO 4 ·7H 2 O Prepare iron-containing PDA comprehensive medium with iron concentrations of 150, 200, 250, 300, 320, 350, 400, 450 mg / L; the raw materials used include: 20 g of potato juice, 20 g of cottonseed hulls, KH-- 2 PO 4 0.3 g, MgSO 4 0.15 g, 2 g agar, H 2 O 100 ml, the pH does not need to be adjusted, it is in a natural state, put it in an autoclave, and sterilize it at 120~130°C for 15 minutes;

[0037] 4) Inserting the strains screened in step 2) into the PDA comprehensive culture medium wit...

Embodiment 2

[0046] Example 2: 10 L iron-containing (FeSO 4 ·7H 2 O) Submerged fermentation of Xinke No. 5 liquid at 2100 mg / L

[0047] 1) Insert Xinke No. 5 bacteria purchased from Zhejiang Qiming Edible Mushroom Co., Ltd. into the sterilized PDA comprehensive medium for in-vitro culture;

[0048] 2) The test tube strains were inserted into the PDA comprehensive culture medium with an iron content of 100 mg / L for cultivation, and iron-resistant strains were screened out;

[0049] 3) with FeSO 4 ·7H 2 O Prepare iron-containing PDA comprehensive medium with iron concentrations of 150, 200, 250, 300, 320, 350, 400, 450 mg / L, respectively. The raw materials used include: 20 g of potato juice, 20 g of cottonseed hulls, KH-- 2 PO 4 0.3 g, MgSO4 0.15 g, agar 2 g, H 2 O 100 ml. The pH does not need to be adjusted, it is in a natural state, put it in an autoclave, and sterilize it at 120~130°C for 15 minutes;

[0050] 4) Inserting the strains screened in step 2) into the PDA comprehensiv...

Embodiment 3

[0059] Embodiment 3: 30 L containing iron (FeSO 4 ·7H 2 O) Submerged fermentation of Xinke No. 5 liquid at 2100 mg / L

[0060] 1) Insert Xinke No. 5 bacteria purchased from Zhejiang Qiming Edible Mushroom Co., Ltd. into the sterilized PDA comprehensive medium for in-vitro culture;

[0061] 2) The test tube strains were inserted into the PDA comprehensive culture medium with an iron content of 100 mg / L for cultivation, and iron-resistant strains were screened out;

[0062] 3) with FeSO 4 ·7H 2 O Prepare iron-containing PDA comprehensive medium with iron concentrations of 150, 200, 250, 300, 320, 350, 400, 450 mg / L, respectively. The raw materials used include: 20 g of potato juice, 20 g of cottonseed hulls, KH-- 2 PO 4 0.3 g, MgSO 4 0.15 g, 2 g agar, H 2 O 100 ml. The pH does not need to be adjusted, it is in a natural state, put it in an autoclave, and sterilize it at 120~130°C for 15 minutes;

[0063] 4) Inserting the strains screened in step 2) into the PDA compreh...

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Abstract

The invention relates to iron-rich black fungus and a production method thereof. The production method includes: firstly preparing stock culture; secondly, screening cultures of the black fungus high in iron tolerance; thirdly, domesticating the cultures; fourthly, separating and purifying the cultures; and fifthly performing submerged culture of the black fungus. According to the method, the black fungus is cultured at 25 DEG C and at the rotation speed 120rpm and is subjected to liquid submerged fermentation for 5 days, and mycelia of the black fungus with iron content of 32000mg / kg can be obtained, and biologic iron in the mycelia reaches 90%.

Description

technical field [0001] The invention relates to iron-rich black fungus and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Iron is one of the essential trace elements for the human body. It is widely distributed in the human body, and almost all tissues contain iron. Iron is an important component of hemoglobin, an essential trace element for transporting and exchanging oxygen in the blood, and is also the active center of many enzymes. Iron deficiency will lead to diseases such as anemia. Iron deficiency in adolescents will affect physical and intellectual development and decline in learning ability; iron deficiency in adults will lead to decreased physical strength and reduced immunity. As far as the society is concerned, iron deficiency leads to the reduction of the intelligence and labor ability of the population, affects the quality and competitiveness of the population, and is not conducive to the long-term develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A01G31/00C05G1/00
Inventor 黄阳毛彦军
Owner MICROHARBOR BIOTECH JIANGSU
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