Multi-channel plant root exudate collection system and collection method thereof

Abstract

The invention discloses a multi-channel plant root exudate collection system and a collection method thereof. The system consists of a plurality of same collection devices and a multi-channel peristaltic pump, wherein the top end of a plant culture tank in each collection device is provided with a cover plate; a water outlet at the bottom end of the plant culture tank is connected with a filtering device; the filtering device is then connected with a root exudate adsorption device through the multi-channel peristaltic pump; and the root exudate adsorption device is then connected to the plant culture tank through a pipeline for recovering nutrient solution. The collection method comprises the following steps of performing water culture or quartz sand culture in the plant culture tank to culture plants; and flowing the nutrient solution from the plant culture tank into the adsorption device holding resin through the filtering device along a pipeline under the driving of the peristaltic pump, wherein the nutrient solution flows back into the plant culture tank after the root exudate is adsorbed by the resin. The collection system and the collection method can be used for simultaneously collecting a plurality of sets of plant root exudates, so that the cost, the time and the space are saved; and the system has a good isolation effect from the exterior, so that the accuracy for collecting the root exudates is improved.

Description

[0001] Technical field [0002] The invention relates to an analysis device and a working method, in particular to a plant root exudate collection system and collection method, and belongs to the field of plant, soil and microbiological sciences. Background technique [0003] Plant root exudates are a series of soluble organic substances secreted by plant roots during the growth process. Its main components include small molecular organic acids, amino acids and sugars. Plant root exudates can not only reflect the physiological conditions of plants, but also change the rhizosphere environment of plants, which has been a hot research topic in recent years. However, because these small molecular organic matter in plant root exudates are easily decomposed by light or absorbed and utilized by microorganisms, some root exudates collection systems and methods have also been proposed, but in practical applications, these methods more or less exist. defect. [0004] The root exudate collec...

AI Technical Summary

Problems solved by technology

However, since these small molecular organic substances in plant root exudates are easily decomposed by light or absorbed by microorganisms, some root exudate collection systems and collection methods have also been proposed, but in practical applications, these methods exist more or less. defect

[0004] The root exudate collection systems designed in Chinese patents CN201933091U, CN1963437A, CN1719220A, and CN1482443A all use air pumps to feed gas, but the gas will interfere with the growth of plant roots, and the microorganisms brought in by the air will accelerate the decomposition of root exudates. Affects the authenticity of collected root exudates

In the root exudate collection system designed by Chinese patent CN101598638B, the nutrient solution enters the chromatographic column for adsorption and collection under the action of gravity, but the collection speed is slow, the collection efficiency is low, and the flow rate is not controlled

The root exudate collection system designed by Chinese patent CN202083567U is an open system, and external factors (such as light, microorganisms, etc.) have great interference on the collection of root exudates

[0005] Existing root exudate collection systems often require a separate pump for each device as a power source for nutrient solution circulation, and a set of devices can only be used for one plant's growth conditions (such as nutrient solution concentration), so the For large-scale conditional experiments, the cost, power consumption and space occupation of the required devices will be very considerable

On the other hand, the amount of root exudates will vary greatly in different stages of plant growth and under different growth conditions. Therefore, under different conditions, a fixed circulation flow rate is used to collect root exudates. Or the authenticity will be greatly affected

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