Oil-enriched marine microalga and application thereof
An application and technology of Nannochloropsis, which is applied in the field of marine microalgae screening and can solve problems such as lack of algae strains
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[0010] The present invention will be described in detail below in conjunction with the examples.
[0011] 1. Screening and physical and chemical properties of Nannochloropsis HH-1 (Nannochloropsis sp.)
[0012] 1. Screening of Nannochloropsis HH-1 (Nannochloropsis sp.)
[0013] The seawater samples taken from the sewage outfall of Shilaoren Beach in Qingdao were picked out under an inverted microscope with a capillary to pick out a single algal cell, and then cultured in a 96-well plate. The medium used for the culture was f / 2 medium (Guillard and Ryther 1962, Guillard 1975). Add the following components to each L of filter-sterilized seawater for f / 2 medium:
[0014] Element
Amount added
NaNO 3
75g / L dH 2 o
1mL
NaH 2 PO 4 ·H 2 o
5g / L dH 2 o
1mL
trace elements
see (1)
1mL
vitamins
See (2)
0.5mL
[0015] (1) Trace element stock solution (the following components sh...
PUM
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