Fermentation culture medium of dextranase-producing bacteria and culture method thereof
A technology of dextranase and fermentation medium, which is applied in the field of bioengineering and can solve the problems of low fermentation level and low fermentation enzyme activity unit.
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Embodiment 1
[0017] Fermentation medium formula 1 with peanut cake powder added: 0.5% peptone, K 2 HPO 4 0.5%, KCl 0.02%, FeSO 4 0.002%, peanut cake powder 0.1%, dextran 3.0%, and the balance is well water.
[0018] Fermentation medium control group 1 without peanut cake powder: peptone 0.5%, K 2 HPO 4 0.5%, KCl 0.02%, FeSO 4 0.002%, dextran 3.0%, and the balance is well water.
[0019] Two groups of small experiments were carried out in a 50L fermenter. Sand spores were inoculated in the slant medium at a temperature of 28°C and a humidity of 40-60% for 5-6 days; mature slant spores were inserted into the shake flask seed medium at a temperature of Cultivate for 40-60 hours at 28°C, humidity 40-60%, rotation speed 220-240RPM; inoculate shake flask seeds at 1% inoculation amount in seed tanks, and cultivate at 28°C for 40-60 hours; inoculate with 1% inoculum amount In the fermentation medium, culture at 28°C for 120-150 hours to end the fermentation.
[0020] The enzyme activity...
Embodiment 2
[0022] Fermentation medium formula 2 with peanut cake powder added: 0.3% peptone, K 2 HPO 4 0.4%, KCl 0.015%, FeSO 4 0.0015%, peanut cake powder 0.2%, dextran 2.8%, and the balance is well water.
[0023] Fermentation medium control group 2 without peanut cake powder: peptone 0.3%, K 2 HPO 4 0.4%, KCl 0.015%, FeSO 4 0.0015%, dextran 2.8%, and the balance is well water.
[0024] Two groups of small experiments were carried out in a 50L fermenter. Sand spores were inoculated in the slant medium at a temperature of 28°C and a humidity of 40-60% for 5-6 days; mature slant spores were inserted into the shake flask seed medium at a temperature of Cultivate for 40-60 hours at 28°C, humidity 40-60%, rotation speed 220-240RPM; inoculate shake flask seeds at 1% inoculation amount in seed tanks, and cultivate at 28°C for 40-60 hours; inoculate with 1% inoculum amount In the fermentation medium, culture at 28°C for 120-150 hours to end the fermentation.
[0025] The enzyme acti...
Embodiment 3
[0027] Fermentation medium formula 3 with peanut cake powder added: 0.2% peptone, K 2 HPO 4 0.3%, KCl 0.01%, FeSO 4 0.001%, 0.5% peanut cake powder, 2.5% dextran, and the balance is well water.
[0028] Fermentation medium control group 3 without peanut cake powder: peptone 0.2%, K 2 HPO 4 0.3%, KCl 0.01%, FeSO 4 0.001%, dextran 2.5%, and the balance is well water.
[0029] Two groups of small experiments were carried out in a 50L fermenter. Sand spores were inoculated in the slant medium at a temperature of 28°C and a humidity of 40-60% for 5-6 days; mature slant spores were inserted into the shake flask seed medium at a temperature of Cultivate for 40-60 hours at 28°C, humidity 40-60%, rotation speed 220-240RPM; inoculate shake flask seeds at 1% inoculation amount in seed tanks, and cultivate at 28°C for 40-60 hours; inoculate with 1% inoculum amount In the fermentation medium, culture at 28°C for 120-150 hours to end the fermentation.
[0030] The enzyme activity...
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