Method for preparing mulberry leaf 1-deoxynojirimycin extract with filter membrane and resin

A deoxynojirimycin and extract technology, applied in the direction of organic chemistry, to achieve the effects of cost reduction, high extraction rate, and increase in mass concentration

Active Publication Date: 2012-10-10
ZHEJIANG JINGYUETANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation of 1-deoxynojirimycin extract from mulberry leaves by using microfiltration and ultrafiltra

Method used

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  • Method for preparing mulberry leaf 1-deoxynojirimycin extract with filter membrane and resin
  • Method for preparing mulberry leaf 1-deoxynojirimycin extract with filter membrane and resin
  • Method for preparing mulberry leaf 1-deoxynojirimycin extract with filter membrane and resin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Crush the dried mulberry leaves, pass through a 100-mesh sieve, mix the mulberry leaf powder and deionized water at a mass ratio of 1:8, reflux and extract at 100°C for 0.5h, filter to obtain the extract and filter cake, and filter the cake Extract again under the same conditions for 0.5h, combine all extracts, concentrate the extract into extract, obtain concentrate 50.0g, i.e. mulberry leaf water extract extract, the mass content of DNJ in the mulberry leaf water extract extract is 1.32%.

[0032] (2) Weigh 50.0g of the concentrate obtained in step (1), dissolve it in 5L of deionized water and prepare a mixed solution with a concentration of 10.0g / L, and use absorbent cotton as a medium for suction filtration to obtain 5L of filtrate; The 0.2μm alumina ceramic membrane was subjected to microfiltration and clarification treatment under the condition of 2bar, the permeate was collected, and deionized water was added to the retentate and then eluted twice (that is, w...

Embodiment 2

[0039] In step (3), a polyethersulfone membrane with a pore size of 5 kDa was used, the concentration of the ammonia solution in step (5) was 0.3 mol / L, and other operations were the same as in Example 1. The mass content of DNJ in the final mulberry leaf DNJ extract was 46.52%, and the extraction rate was 13.43%.

Embodiment 3

[0041] In step (3), a polyethersulfone membrane with a pore size of 10 kDa was used, and the concentration of the ammonia solution in step (5) was 0.8 mol / L. Other operations were the same as in Example 1. The mass content of DNJ in the final mulberry leaf DNJ extract was 46.14%, and the extraction rate was 13.21%.

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Abstract

The invention discloses a method for preparing mulberry leaf 1-deoxynojirimycin extract with a filter membrane and resin. The method includes dissolving aqueous extract of mulberry leaves; sequentially performing microfiltration and ultrafiltration; and treating permeate liquor by macroporous adsorption resin, a strongly acidic cation exchange resin column and a strongly basic anion exchange resin column to obtain the mulberry leaf 1-deoxynojirimycin extract. DNJ (deoxynojirimycin) in mulberry leaves is enriched and purified by the membrane separation technology combining ultrafiltration and microfiltration, the process is simple and efficient, any organic solvents are omitted, and the method is safe and environment-friendly. The mass concentration of DNJ in the obtained mulberry leaf DNJ extract is improved from 1.32% to 7.84% as compared with that of DNJ in the aqueous extract of the mulberry leaves, and the extraction ratio is 88.06%.

Description

(1) Technical field [0001] The invention relates to the technical field of separation of effective components of traditional Chinese medicine, in particular to a method of enriching and purifying 1-deoxynojirimycin (DNJ) in mulberry leaves by using membrane separation technology and resin method to prepare mulberry leaf 1-deoxynojirimycin extract Methods. (2) Background technology [0002] Mulberry leaf, also known as iron fan in traditional Chinese medicine, is the leaf of the plant mulberry (Morus alba L.). First recorded in "Shen Nong's Materia Medica", it tastes bitter, sweet, and cold in nature, and returns to the lung and liver meridians. It has the effects of dispelling wind and clearing heat, calming the liver and improving eyesight. It is recorded in Chinese medicine books of past dynasties that mulberry leaves can treat diabetes, and modern doctors often use mulberry leaves to treat diabetes, and have achieved satisfactory results. Modern pharmacological studies ...

Claims

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Application Information

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IPC IPC(8): C07D211/46
Inventor 颜继忠李行诺雷伟
Owner ZHEJIANG JINGYUETANG PHARMA
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