Method for detecting quality of alkaloids in Tibetan medicine herba aconiti tangutici and preparations thereof

A detection method and preparation technology, which is applied in the field of quality detection of alkaloids in the Tibetan medicine Aconitum tanguta and its preparations, can solve problems such as hidden health risks and difficulty in ensuring safe and effective use of medicinal materials

Active Publication Date: 2015-06-17
SHANDONG JINHE DRUG RES DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The whole herb of Aconitum tangutica is used as medicine. The height of the herb is 10-30cm, the tuber is 2, and small in shape. It can be used as medicine after concocting, but overdose may bring health risks
Tangut Aconitum is recorded in the "Standard issued by the Ministry of Tibetan Medicine". The name of Tibetan medicine is Bangga. The quality of its medicinal materials is difficult to guarantee the safety and effectiveness of medicinal materials, so it is necessary to establish a scientific quality detection method to evaluate the quality of Aconitum tanguta and its preparations

Method used

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  • Method for detecting quality of alkaloids in Tibetan medicine herba aconiti tangutici and preparations thereof
  • Method for detecting quality of alkaloids in Tibetan medicine herba aconiti tangutici and preparations thereof
  • Method for detecting quality of alkaloids in Tibetan medicine herba aconiti tangutici and preparations thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0054] Experimental Example 1: TLC identification experiment of Aconitum tanguticum

[0055] Aconitum tanguticum was provided by Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.

[0056] TLC identification:

[0057] Take 1g of the medicinal powder to be tested (passed through No. 3 sieve), add 5ml of ammonia water, add 50ml of dichloromethane, ultrasonic treatment (power 250W, frequency 40kHz) for 30 minutes, filter, evaporate the filtrate, and add 1ml of methanol to the residue to dissolve it. The test solution. Take 1g of the control medicinal material of Aconitum tanguticum, and prepare the control medicinal material solution in the same way. According to thin layer chromatography (Appendix VI B of the 2010 edition of the People's Republic of China Pharmacopoeia), draw 10μl of each of the above two solutions, and spot them on the same silica gel G thin layer plate, with a volume ratio of 5:4: 1.5:0.2 n-hexane-ethyl acetate-methanol-ammonia water as a developing agent, un...

experiment example 2

[0059] Experimental example 2: Examination of diester alkaloids in Aconitum tanguticum

[0060] 1. Instruments, reagents and test samples

[0061] Instrument: High performance liquid chromatograph: Hitachi L-2100 pump, Hitachi L-2400 UV detector;

[0062] Electronic balance: Shimadzu AUW220D type.

[0063] Reference substance: aconitine reference substance (China Institute for the Control of Pharmaceutical and Biological Products), batch number: 110720-200410; subaconitine (China National Institute for the Control of Pharmaceutical and Biological Products), batch number: 110798-200805; new aconitine (China Pharmaceutical and Biological Products) Product Inspection Institute), batch number: 110799-200505.

[0064] Sample: Aconitum tanguticum (provided by Qinghai Jinhe Tibetan Medicine Co., Ltd.), batch numbers are 110701, 110702, 110703.

[0065] 2. Selection of detection wavelength

[0066] Take aconitine reference substance, subaconitine reference substance, and new aconitine reference ...

experiment example 3

[0123] Experimental Example 3: Determination of Hordeine in Aconitum tanguticum

[0124] 1. Instruments, reagents and test samples

[0125] Instrument: High performance liquid chromatograph: Hitachi L-2100 pump, Hitachi L-2400 UV detector;

[0126] Electronic balance: Shimadzu AUW220D type.

[0127] Reference substance: Hordeine reference substance (China Institute for the Control of Pharmaceutical and Biological Products), batch number: 100762-200901.

[0128] Sample: Aconitum tanguticum (provided by Qinghai Jinhe Tibetan Medicine Co., Ltd.), batch numbers are 110701, 110702, 110703.

[0129] 2. Selection of detection wavelength

[0130] Take the hordeine reference substance solution, scan in the wavelength range of 190-400nm, and select 225nm as the detection wavelength according to the ultraviolet absorption spectrum.

[0131] 3. Selection of mobile phase and column

[0132] The acetonitrile-volume ratio is 0.5% triethylamine aqueous solution (pH3.0 adjusted by phosphoric acid), acetonit...

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Abstract

The invention discloses a method for detecting quality of alkaloids in a Tibetan medicine herba aconiti tangutici and preparations thereof. The method comprises the steps of identification, check and content determination, wherein thin layer chromatography is adopted for identification and is characterized by taking a herba aconiti tangutici control medicine as the control and normal hexane-ethyl acetate-methanol-ammonia water as a developer; high performance liquid chromatography is adopted for check and is characterized by taking a mixed solution of an aconitine control substance, a hypaconitine control substance and a new aconitine control substance as the control, acetonitrile-tetrahydrofuran as a mobile phase A and an ammonium acetate water solution with glacial acetic acid as a mobile phase B to carry out gradient elution; high performance liquid chromatography is adopted for hordenine content determination and is characterized by taking a hordenine control substance as the control and an acetonitrile-potassium dihydrogen phosphate water solution as a mobile phase; and back titration is adopted for total alkaloid content determination. The method is stable and reliable, has strong specificity, can ensure the safety, effectiveness and quality controllability of the alkaloids in the Tibetan medicine herba aconiti tangutici and the preparations thereof, and has higher scientificity and application values.

Description

Technical field [0001] The invention relates to a quality detection method, in particular to a quality detection method of alkaloids in the Tibetan medicine Tanggute Aconitum and its preparation. The method includes the quality detection method of alkaloids in Tanggute Aconitum and its preparation Baweizhangyacai pills, alkaloids in eleven flavor golden pills, Shisanwei Bangga San, and Anerning granules . Background technique [0002] Herba Aconiti Tangutici (Herba Aconiti Tangutici) is the dry whole plant of the Ranunculaceae plant Aconitum tanguticum (Maxim.) Stapf and Aconitum naviculare (Bruhl.) Stapf. At the end of summer and early autumn, the roots are excavated to remove impurities and dry in the shade. This product is cool in nature, bitter in taste, has the effects of clearing away heat, detoxifying, regenerating muscles and closing mouth, and drying dampness. It is mainly used for heat-clearing and detoxification, mainly used for liver fever, gallbladder fever, lung ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/02G01N31/16G01N30/36G01N30/88
Inventor 邵成雷王海苹李丽任松鹏单玉刚刘玉芹郑亭亭
Owner SHANDONG JINHE DRUG RES DEV
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