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Method for researching photosynthesis through combination of chlorophyll fluorescence technique and protoplast system, and application thereof

A chlorophyll fluorescence, protoplast technology, applied in the field of scientific research, to achieve the effect of convenient research

Inactive Publication Date: 2015-04-29
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] However, so far, there is no report on the method for studying photosynthesis by measuring the chlorophyll fluorescence of Arabidopsis mesophyll cell protoplast transient expression system

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  • Method for researching photosynthesis through combination of chlorophyll fluorescence technique and protoplast system, and application thereof
  • Method for researching photosynthesis through combination of chlorophyll fluorescence technique and protoplast system, and application thereof
  • Method for researching photosynthesis through combination of chlorophyll fluorescence technique and protoplast system, and application thereof

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Embodiment Construction

[0041] Below in conjunction with experiment, further illustrate the present invention.

[0042] 1. Establishment of protoplast chlorophyll fluorescence measurement system

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Abstract

The invention discloses a method for researching photosynthesis through combination of a chlorophyll fluorescence technique and a protoplast system, and application thereof. Genes related to photosynthesis can be screened at high throughput quickly through instantly expressing or silencing one or more of genes in a protoplast, determining change of chlorophyll fluorescence of the protoplast of an arabidopsis mesophyll cell, and comparing an image thereof.

Description

technical field [0001] The invention relates to a method for scientific research, in particular to a method for studying photosynthesis. Background technique [0002] The growth and development of plants is inseparable from photosynthesis. Photosynthesis is the material basis of all material metabolism and energy metabolism in the biological world. It includes a series of complex processes of photophysical, photochemical and biochemical transformations. The research on photosynthesis will help people understand the process of photosynthesis more deeply, and then carry out transgenic treatment on plants, especially crops, to improve the efficiency of photosynthesis. [0003] At present, the study of the function of genes involved in photosynthesis is mainly through stable transformation to obtain overexpression, interference with plants, or through mutants, which takes a long period of time and a lot of work. This research method is inefficient, and it is difficult to effici...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/02G01N21/64
Inventor 王宏斌苏建斌段珊刘兵冯冬茹王金发
Owner SUN YAT SEN UNIV
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