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Methods and compositions related to reduced met phosphorylation by leukocyte cell-derived chemotaxin 2 in tumor cells

A tumor cell, phosphorylation technology, applied in the field of inhibition of tumor cell proliferation, phosphorylation, prevention or treatment of hepatocellular carcinoma, pharmaceutical composition for prevention or treatment of tumors, migration and invasion, and can solve unreported problems

Inactive Publication Date: 2012-11-28
TTY BIOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the exact role of LECT2 in cancer development such as migration, invasion and metastasis has not been reported yet

Method used

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  • Methods and compositions related to reduced met phosphorylation by leukocyte cell-derived chemotaxin 2 in tumor cells
  • Methods and compositions related to reduced met phosphorylation by leukocyte cell-derived chemotaxin 2 in tumor cells
  • Methods and compositions related to reduced met phosphorylation by leukocyte cell-derived chemotaxin 2 in tumor cells

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Experimental program
Comparison scheme
Effect test

Embodiment

[0194] Materials and Methods

[0195] cell culture

[0196] All cancer cell lines used were obtained from the American Type Culture Collection (Rockville, Maryland, USA).

[0197] Hepatocellular carcinoma cells were grown in DMEM medium (Life Technologies, GIBCO BRL, Rockville, Maryland) at 37°C in a humidified atmosphere of 5% carbon dioxide-95% air, wherein the medium had 10 % fetal bovine serum albumin (FBS) and 2 ml of L-glutamine (Life Technologies). Cells were cultured according to the supplier's recommendations. Adherent cells were detached from the dishes by trypsin-EDTA treatment.

[0198] A549 (human lung adenocarcinoma) was maintained and cultured in DMEM medium. CL1-5 (human lung adenocarcinoma), MB-MDA231 (human breast cancer), N87 (human gastric cancer) were maintained and cultured in RPMI-1640 medium. All media were supplemented with 10% FBS.

[0199] Transfection and establishment of stable transfected cells

[0200] The expression vector of LECT2 was es...

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Abstract

It was discovered that leukocyte cell-derived chemotaxin 2 (LECT2) correlated with down-regulated vascular invasion in HCC patients. It was also found that LECT2 strongly reduced the growth, migration and invasiveness of HCC cells via inhibition of the phosphorylation of Met and other downstream targets in the HGF / MET pathway. The HXXXD motif of LECT2 was found to be important for its tumor inhibition mechanism. LECT2 reduced Met tyrosine phosphorylation and tumor cell invasion in other cancers, such as lung, breast, and gastric cancers, in addition to HCC. Methods and compositions for preventing, treating or diagnosing tumors, such as HCC, based on the newly discovered tumor suppression property of LECT2 are described.

Description

[0001] Cross References to Related Applications [0002] Pursuant to 35 U.S.C. §119(e), this application is granted priority to U.S. Provisional Patent Application No. 61 / 288,627, filed December 21, 2009. Background technique [0003] Hepatocellular carcinoma (HCC) is one of the most prevalent forms of cancer worldwide, with an annual incidence of approximately one million cases (1). In Taiwan, hepatocellular carcinoma continues to be the leading cause of cancer-related death in men and the second leading cause of cancer-related death in women. The five-year survival rate for higher stages of HCC, such as stages II to III, is about 20%, which is less than a third of that for stage I (ie, about 60%). High cancer recurrence remains the leading cause of death in HCC patients. Major adverse prognostic factors include vascular invasion (2). High-throughput suppression subtractive hybridization (SSH, suppression subtractive hybridization) using human membrane / secretory protein (...

Claims

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Application Information

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IPC IPC(8): A61K38/19A61P35/00
CPCA61K38/19A61K38/195A61P35/00
Inventor 郭明良吴玉琳
Owner TTY BIOPHARM
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