Human breast cancer chronic intermittent hypoxia cell line, and preparation method thereof

A human breast cancer, intermittent technology, applied in tumor/cancer cells, biochemical equipment and methods, animal cells, etc., can solve the lack of in vivo experimental support, the current status of chronic intermittent hypoxia cell line culture can not meet the hypoxia related Research and other issues to achieve the effect of fast proliferation, strong invasive ability, and improved quality level

Inactive Publication Date: 2012-12-19
FUDAN UNIV SHANGHAI CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009]2. Lack of in vivo experimental support
[0011]In view of this, the current culture status of chronic intermittent hypoxia cell lines is far from meeting the needs of hypoxia-related research

Method used

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  • Human breast cancer chronic intermittent hypoxia cell line, and preparation method thereof
  • Human breast cancer chronic intermittent hypoxia cell line, and preparation method thereof
  • Human breast cancer chronic intermittent hypoxia cell line, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 establishes MCF-7H cell line

[0031] The human breast cancer cell line MCF-7 was used as the source cell, and the in vitro culture method of chronic intermittent hypoxia was used for passage, and a stable growth cell line of chronic intermittent hypoxia was established, and the human breast cancer chronic intermittent hypoxia cell line MCF was obtained. -7H, has been preserved on May 20, 2011, preservation unit: General Microbiology Center (CGMCC), China Committee for the Collection of Microbial Cultures, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences , deposit number: CGMCC No.4902, classification designation: human breast cancer cells, MCF-7H.

[0032]In this example, the source cell human breast cancer cell line MCF-7 was cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 100 IU / ml penicillin and 100 μg / ml streptomycin; The hypoxia period of 1 hour and th...

Embodiment 2

[0035] Example 2 Human breast cancer chronic intermittent hypoxia cell line MCF-7H in vitro and in vivo culture, experiment

[0036] The results of in vitro culture of the human breast cancer chronic intermittent hypoxia cell line MCF-7H of the present invention show that the cell line has the following biological characteristics: good growth in vitro culture, passage once every 3 days; cells in multipolar epithelial morphology Features, aggregated into sheets or clusters, abundant cytoplasm, adherent growth, loss of contact growth inhibition, no obvious difference in morphology from the source cells (such as figure 1 shown); the doubling time of the cell line was about 8 hours, while the doubling time of the source cells was about 27 hours. The cell growth curve traced by the CCK-8 method suggested that the proliferation rate of MCF-7H cells was significantly faster than that of the source cells (P figure 2 shown); the results of Transwell invasion assay confirmed that MCF-...

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Abstract

The invention belongs to the field of microbial and animal cell lines, and relates to a human breast cancer chronic intermittent hypoxia cell line. Stable-growth human breast cancer chronic intermittent hypoxia cell strain MCF-7H is obtained by employing human breast cancer cell line MCF-7 as stem cells and by using chronic intermittent hypoxia in-vitro culture for passage. The preservation number of the cell strain MCF-7H is CGMCC No. 4902; and the classification name is human breast cancer chronic intermittent hypoxia cell strain MCF-7H. The cells show multi-polar epithelial morphological characteristics, aggregate into sheet or cluster shapes, have fast multiplication rate, strong invasion capability and up-regulated expression of regulatory pathway of a hypoxia reaction, and have tumor formation capability not significantly different from that of the stem cell line. The formed transplanted tumor has fast growth rate; and simultaneously expression of regulatory pathway of the hypoxia reaction is up-regulated continuously. The human breast cancer chronic intermittent hypoxia cell line can reproduce the human breast cancer cell line produced in a real in-vivo hypoxia environment, and is an ideal and reliable model for in-vivo and in-vitro hypoxia-related researches.

Description

technical field [0001] The invention belongs to the field of microbial animal cell lines, and relates to a human breast cancer chronic intermittent hypoxia cell line and a preparation method thereof, in particular to a human breast cancer chronic intermittent hypoxia cell line MCF-7H. Background technique [0002] Hypoxia is a ubiquitous phenomenon in the development of malignant solid tumors, including breast cancer. Hypoxia will cause some tumor cells to die and form necrotic foci, but most tumor cells can still grow. In order to adapt to or overcome the hypoxic environment, a series of biochemical and pathological changes will occur inside the tumor tissue, called the hypoxic response, which mainly includes the increase of anaerobic metabolism, the inhibition of apoptosis, the formation of new blood vessels, the local tumor infiltration and Enhanced transfer ability, etc. [0003] It is currently believed that the hypoxia response is initiated and regulated by the hypo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12R1/91
Inventor 邵志敏柳光宇殷文瑾雷莉罗建民欧周罗吴炅沈镇宙
Owner FUDAN UNIV SHANGHAI CANCER CENT
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