Equipment and method for identifying salt tolerance of plant at seedling stage

A technology for salt tolerance and plant seedlings is applied in the field of identification equipment for salt tolerance in the seedling stage of plants, which can solve problems such as difficult salt content and affect root growth, and achieve the effects of improving air permeability, convenient operation, and uniform seedlings.

Inactive Publication Date: 2013-01-23
ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a solution for the defects of the traditional salt solution identification method at the seedling stage that it is difficult to maintain the relatively constant salinity in the pot and the uniformity between different pots, and the lack of oxygen affecting the growth of the root system after the plant root system has been immersed in the salt solution for a long time. A kind of equipment and method that can keep the salt content in the pot relatively constant and uniform among different pots during the test period, and ensure the oxygen demand of the root system to identify the salt tolerance of the plant at the seedling stage

Method used

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  • Equipment and method for identifying salt tolerance of plant at seedling stage
  • Equipment and method for identifying salt tolerance of plant at seedling stage
  • Equipment and method for identifying salt tolerance of plant at seedling stage

Examples

Experimental program
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Embodiment 1

[0033] Example 1: (a device 1 for identifying the salt tolerance of plant seedlings)

[0034] The equipment is made of tin plate or stainless steel plate, specifically composed of tank 1, partition 2, left tank 3, right tank 4, sieve filler A5, sieve filler B6, water inlet A7, water inlet B8, salt solution Storage tank 9, ball float valve A10, water storage tank 11, ball float valve B12, drain pipe A13, drain pipe B14, micro water pump A15, micro water pump B16, timer 17, DC regulator 18, power plug 19, waste liquid tank 20 components; wherein, the water tank 1 is a square or rectangular shape with a height of 7cm on all sides, and in its middle part is provided with a vertical wall vertical to the bottom surface and the upper and lower ends and equal to the height of the four edges. Partition board 2, the partition board 2 separates the water tank 1 into a left water tank 3 and a right water tank 4 which are not leaking each other, and a sieve-shaped filling plate A5 and a si...

Embodiment 2

[0035]Example 2: (a device 2 for identifying the salt tolerance of plant seedlings)

[0036] In this example, the water tank 1 is molded from engineering plastics; other parts and structures are the same as those in Example 1.

Embodiment 3

[0037] Embodiment 3: (a kind of method 1 of identification plant seedling salt tolerance)

[0038] A method for using the equipment of claim 1 to identify plant seedling salt tolerance, the method is carried out as follows:

[0039] 1) Cultivation of plug seedlings of plants to be identified: select a 50-hole plug tray 21 with a length, width, and height of 54×28×5 cm, fill each planting hole of the plug tray with a mixture of peat and coarse sand in a volume ratio of 2:1, and sow seeds in each hole 1-3 grains, after the emergence of a pair of true leaves, thin the seedlings and fill in the gaps, so that there is a seedling in each hole of the plug plate, for use;

[0040] 2) Preparation of salt solution for identification: prepare NaCl and water to make a salt solution with a concentration of 0.4% and store it in the salt solution storage tank 9; in addition, store clean water in the clean water storage tank 11 for standby;

[0041] 3) The combination of plug seedlings and s...

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Abstract

The invention discloses equipment and a method for identifying the salt tolerance of a plant at the seedling stage, and belongs to the technical field of plant salt tolerance identification. The method comprises the following steps of: 1) breeding plug seedlings of the plant to be identified; 2) preparing a saline solution for identification; 3) combining the plug seedlings with the salt tolerance identification equipment; 4) deeply soaking the plug seedlings into the solution; 5) regularly changing the solution; and 6) performing the identification and evaluation on the seedling-stage salt tolerance of the plant, and the like. As the treatment conditions for the seedlings to be identified and the reference seedlings are the same and the liquid level and concentration of the saline solution are kept stable, the equipment and the method provided by the invention are simple to operate, high in identification efficiency, good in comparison property and low in test error; and therefore, the accuracy and reliability of identification on the seedling-stage salt tolerance of the plant are improved. The equipment and the method provided by the invention can be popularized and applied to agricultural research institutions and seed enterprises.

Description

technical field [0001] The invention relates to the technical field of identification of plant salt tolerance, in particular to equipment and methods for identification of plant seedling salt tolerance. Background technique [0002] Plant salt-tolerance identification technology has been widely used in germplasm resource identification, salt-tolerant variety breeding and salt-tolerant physiological research. There are many methods for salt tolerance identification, such as germination index method, seedling salt solution identification method, nutrient solution method, tissue culture method and field identification method, etc. (Zhang Yongyin, Kou He, Zhao Fucai, etc. Evaluation methods for crop salt tolerance. Journal of Liaoning Agricultural Vocational and Technical College. 2009 (3) 6 ~ 7); The above methods have their own advantages and disadvantages. In practice, the salt solution identification method at the seedling stage is often used. This method is usually to cult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
Inventor 潘刚敏王炜勇沈晓岚俞少华葛亚英田丹青
Owner ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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