Dynamic two-dimensional preparative liquid chromatography system and substance separating and purifying method

A two-dimensional preparation and liquid chromatography technology, applied in the direction of material separation, material analysis, measuring devices, etc., can solve the problem of high investment, achieve a wide range of applications, reduce hardware and production costs, and be easy to automate

Active Publication Date: 2014-08-13
中科沃业江苏生物有限公司
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Problems solved by technology

However, the online two-dimensional liquid chromatography system requires two preparative liquid chromatography systems. For the separation and purification of a single target in complex samples, the initial investment is too high

Method used

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  • Dynamic two-dimensional preparative liquid chromatography system and substance separating and purifying method
  • Dynamic two-dimensional preparative liquid chromatography system and substance separating and purifying method
  • Dynamic two-dimensional preparative liquid chromatography system and substance separating and purifying method

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Embodiment Construction

[0030] As mentioned above, in view of many defects in the prior art, the inventors of the present case provided a quasi-dynamic two-dimensional preparative liquid chromatography system and a substance separation and purification method based on the quasi-dynamic two-dimensional preparative liquid chromatography system.

[0031] As an aspect of the present invention, its technical scheme is as follows:

[0032] A quasi-dynamic two-dimensional preparative liquid chromatography system, including a first-dimensional chromatography unit and a second-dimensional chromatography unit, the first-dimensional chromatography unit and the second-dimensional chromatography unit are connected by a three-way valve to form a quasi-dynamic two-dimensional preparative liquid chromatography System for large-scale preparation and production. Wherein, the first-dimensional chromatographic unit may be a set of one-dimensional chromatographic analysis system, including a first-dimensional chromatogra...

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Abstract

The invention discloses a dynamic two-dimensional preparative liquid chromatography system and a substance separating and purifying method. The dynamic two-dimensional preparative liquid chromatography system comprises a first dimension chromatography unit, a second dimension chromatography unit and a three-way valve, wherein the first dimension chromatography unit comprises a first dimension chromatographic column, a sample inlet unit and a detection unit, the second dimension chromatography unit comprises a second dimension chromatographic column, a sample inlet end and a sample outlet end of the first dimension chromatography column are respectively connected with the sample inlet unit and a first connector of the three-way valve, a sample inlet end and a sample outlet end of the second dimension chromatography column are respectively connected with a second connector of the three-way valve and the detection unit, and the third connector of the three-way valve is communicated with the detection unit. By switching combination states of the three-way valves, the first dimension chromatography unit independently works or cooperatively works with the second dimension chromatography unit. Large sample inlet quantity can be guaranteed, system separation capability is improved greatly, hardware and production cost is greatly reduced, and the dynamic two-dimensional preparative liquid chromatography system is wide in application range and particularly applicable to separation and purification of microcomponents in complicated samples.

Description

technical field [0001] The invention specifically relates to a quasi-dynamic two-dimensional preparative liquid chromatography system and a material separation and purification method, belonging to the technical field of chromatographic separation. Background technique [0002] Preparative liquid chromatography is a high-efficiency separation technology for obtaining pure samples. It was born in the 1930s and was first used in the separation of pigments. It has made great progress and has been widely used in the pharmaceutical industry and protein separation and purification. , such as the separation and purification of secondary metabolites, natural products, macromolecular compounds and some important pharmaceutically active proteins, biotherapeutics and diagnostic reagents. However, compared with analytical high-performance liquid chromatography, the separation efficiency of preparative liquid chromatography is not high, and it cannot meet the needs of high purity and hig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/46
Inventor 张维冰邵平戈兆松刘翻张权青
Owner 中科沃业江苏生物有限公司
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