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Method for detecting content difference of aspartyl-enzyme of asparagus plant

A technology of asparaginase and a detection method, which is applied in the field of detection of differences in asparaginase content, can solve problems such as differences in asparaginase content, and achieve the effects of alleviating the shortage of supply and improving efficiency

Inactive Publication Date: 2013-02-27
BEIJING UNIV OF AGRI
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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a method for detecting the difference in asparaginase content of the plant of A. chinensis, which can detect the difference in the content of asparaginase in different parts of the plant of A. chinensis. The problem of detecting the difference of asparaginase content in Shi Diaobo

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  • Method for detecting content difference of aspartyl-enzyme of asparagus plant
  • Method for detecting content difference of aspartyl-enzyme of asparagus plant
  • Method for detecting content difference of aspartyl-enzyme of asparagus plant

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Embodiment Construction

[0012] The following clearly and completely describes the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0013] The embodiment of the present invention provides a method for detecting the difference in the content of asparaginase in cypress plants, comprising the following steps:

[0014] Extract the cypress plant, take the root, stem of the plant (the stem can be a material with a length of 20cm from the top to the bottom of the stem and an average diameter of 0.5-1.2cm at the base, which can be the tender stem commonly referred to in the industry) and leaves as The samples were made into sample solutions respectively, and the sample ...

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Abstract

The invention discloses a method for detecting the content difference of aspartyl-enzyme of an asparagus plant. The method comprises the following steps of: extracting the asparagus plant; taking the root, the stem and leaves of the plant to be used as samples; respectively preparing sample solutions by using the samples; carrying out colorimetric analysis on the sample solutions with reference substance solutions by using a colorimetric method; and calculating optical absorbance values and comparing so as to obtain the difference of the contents of aspartyl-enzyme in different parts of the asparagus plant. By utilizing the method, the root, the stem and the leaves of the asparagus plant are used for preparing the sample solutions and are subjected to the colorimetric analysis by using the colorimetric method, and the difference of the contents of the aspartyl-enzyme at the different parts of the asparagus plant can be accurately compared, so that the accurate basis is provided for extracting the aspartyl-enzyme by using the asparagus plant, the efficiency for extracting the aspartyl-enzyme from the asparagus plant is greatly improved, and the problem that the aspartyl-enzyme is short in market is relieved.

Description

technical field [0001] The invention relates to a method for detecting the difference in the content of asparaginase in philodendron plants. Background technique [0002] Shi Diaobo has a unique flavor, rich nutrition, and anti-cancer and anti-cancer miraculous effects. It is known as the "Vegetable King". This is mainly because it contains a variety of biologically active substances that regulate the physiological functions of the human body and prevent the occurrence of chronic diseases. The asparaginase contained in Shi Diaobo has certain curative effects on breast cancer, lung cancer, esophageal cancer, bladder cancer, skin cancer, gastric cancer, and rectal cancer. However, the asparaginase currently on the market is L-asparaginase prepared by Escherichia coli (E.coli) and Erwinia (Erwinia). There is no report on the relevant detection method. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a method for d...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 韩莹琰范双喜程继鸿任月
Owner BEIJING UNIV OF AGRI
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