Hematopoietic stem cell cryopreserving method and protective agent
A hematopoietic stem cell and protective agent technology, applied in the field of hematopoietic stem cell cryopreservation, can solve the problems of restricting hematopoietic stem cell transplantation, cumbersome operation, high cost, etc., and achieve the effect of easy mastering and popularization, simple equipment and easy operation
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[0013] The present invention will be further described below in conjunction with specific embodiments, rather than limiting the present invention.
[0014] Transfer the sterile blood bag containing the hematopoietic stem cell suspension separated by the blood cell separator or the bone marrow fluid extracted by the operation to the aseptic operation room or ultra-clean bench, choose DMSO, 1640 sterile medium and the blood preservation solution used by the blood cell separator Ⅰ (ACDⅠ) solution is used as stem cell protection agent. The final ratio of the mixture of stem cell protection agent and hematopoietic stem cell suspension or surgically extracted bone marrow fluid is DMSO 9-10%, 1640 sterile medium 5%, ACDⅠ solution 5- 6%, hematopoietic stem cell suspension or bone marrow fluid 79-81%, of which hematopoietic stem cell suspension or bone marrow fluid does not need to be treated with red blood cells. First, pre-mix the three components of the stem cell protection agent DM...
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